温石棉和二氧化硅对苯并(a)芘微粒体代谢的影响。
Effect of chrysotile asbestos and silica on the microsomal metabolism of benzo(a)pyrene.
作者信息
Kandaswami C, O'Brien P J
出版信息
Environ Health Perspect. 1983 Sep;51:311-4. doi: 10.1289/ehp.8351311.
The effects of chrysotile, water-leached chrysotile, and silica on microsomal metabolism of benzo(a)pyrene in vitro were studied. Examination of benzo(a)pyrene metabolites generated by 3-methylcholanthrene-pretreated rat liver microsomes, in the presence of chrysotile fibers, revealed a reduction in the overall metabolism of the hydrocarbon. Thus, chrysotile appeared to modify the activities of aryl hydrocarbon hydroxylase and epoxide hydrolase. Leaching chrysotile in deionized water for 24 hr elicited a similar response. Silica, in contrast to chrysotile, did not decrease the microsomal metabolism of benzo(a)pyrene. Chrysotile, as well as water-leached chrysotile, considerably diminished the microsomal production of water-soluble benzo(a)pyrene metabolites. Precoating the fibers with heparin or bovine serum albumin partially abolished this inhibition. The liver microsomal metabolism of benzo(a)pyrene in rats subjected to intraperitoneal administration of chrysotile was 58% of that in untreated rats.
研究了温石棉、水浸温石棉和二氧化硅对苯并(a)芘体外微粒体代谢的影响。在温石棉纤维存在的情况下，对经3-甲基胆蒽预处理的大鼠肝微粒体产生的苯并(a)芘代谢物进行检测，发现该烃类的总体代谢有所减少。因此，温石棉似乎改变了芳烃羟化酶和环氧化物水解酶的活性。将温石棉在去离子水中浸提24小时引发了类似的反应。与温石棉相反，二氧化硅并未降低苯并(a)芘的微粒体代谢。温石棉以及水浸温石棉显著减少了苯并(a)芘水溶性代谢物的微粒体生成。用肝素或牛血清白蛋白对纤维进行预包被可部分消除这种抑制作用。腹腔注射温石棉的大鼠体内苯并(a)芘的肝微粒体代谢是未处理大鼠的58%。
Zotero 插件