两栖类黑色素细胞的长期培养。体外老化及向连续细胞系的自发转化。
Long-term cultivation of amphibian melanophores. In vitro ageing and spontaneous transformation to a continuous cell line.
作者信息
Kondo H, Ide H
出版信息
Exp Cell Res. 1983 Nov;149(1):247-56. doi: 10.1016/0014-4827(83)90396-8.
Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in growth characteristics in mass culture and in clone culture, by the appearance of the cells, and by measurements of cell volumes. Even after becoming a continuous cell line, amphibian melanophores continued to have a diploid chromosome number (2n = 26) in three of four cell lines examined. The chromosome mode in one cell line, however, changed to thirty. Measurement of melanin dispersion after the addition of alpha-melanocyte-stimulating hormone suggested that the mechanism for melanin dispersion in melanophores changed during in vitro ageing.
从牛蛙蝌蚪的尾部皮肤中分离出的纯黑素细胞群体进行了为期2至3年的大规模培养。研究的所有两栖类黑素细胞系都经历了生长危机（体外老化），随后自发转化为连续细胞系，这通过大规模培养和克隆培养中的生长特性变化、细胞外观以及细胞体积测量得以体现。即使成为连续细胞系后，在所检测的四个细胞系中的三个中，两栖类黑素细胞的染色体数目仍保持二倍体（2n = 26）。然而，其中一个细胞系的染色体模式变为了30。添加α - 黑素细胞刺激激素后对黑色素扩散的测量表明，黑素细胞中黑色素扩散的机制在体外老化过程中发生了变化。
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