AMP与果糖2,6-二磷酸对果糖1,6-二磷酸酶抗胰蛋白酶失活的协同作用。
Synergistic effect of AMP and fructose 2,6-bisphosphate on the protection of fructose 1,6-bisphosphatase against inactivation by trypsin.
作者信息
Han P F, Han G Y, Hayes R L, Moore C L, Johnson J
出版信息
Experientia. 1983 Nov 15;39(11):1305-7. doi: 10.1007/BF01990383.
The rate of inactivation of chicken liver fructose 1,6-bisphosphatase by trypsin is reduced if the digestive reaction is conducted in the presence of AMP or fructose 2,6-bisphosphate. The effects of these 2 compounds are synergistic. Although fructose 1,6-bisphosphate does not protect the enzyme against tryptic inactivation, it can enhance the effect of AMP. Selective modification of the AMP allosteric site of fructose 1,6-bisphosphatase with pyridoxal-P and NaBH4 renders the enzyme more resistant to tryptic inactivation, but the modified enzyme is no longer responsive to the protective effect of AMP.
如果在AMP或果糖2,6 -二磷酸存在的情况下进行消化反应，胰蛋白酶使鸡肝果糖1,6 -二磷酸酶失活的速率会降低。这两种化合物的作用是协同的。虽然果糖1,6 -二磷酸不能保护该酶免受胰蛋白酶失活，但它可以增强AMP的作用。用吡哆醛 - P和硼氢化钠对果糖1,6 -二磷酸酶的AMP别构位点进行选择性修饰，使该酶对胰蛋白酶失活更具抗性，但修饰后的酶不再对AMP的保护作用产生反应。
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