鼠伤寒沙门氏菌LT2菌株中IS200拷贝的基因定位。
Genetic mapping of IS200 copies in Salmonella typhimurim strain LT2.
作者信息
Lam S, Roth J R
出版信息
Genetics. 1983 Dec;105(4):801-11. doi: 10.1093/genetics/105.4.801.
Abstract
The wild-type Salmonella typhimurium strain LT2 contains six copies of the insertion sequence element IS200 which is unique to Salmonella. We have determined the chromosomal locations of all six copies of IS200 in strain LT2. This was done by mapping the positions of Tn10 elements inserted near each copy of IS200. Such Tn10 insertions were detected by Southern hybridization as IS200-containing restriction fragments with altered electrophoretic mobility. The copies are located at quite evenly spaced sites in the chromosome. Some are found in regions with many known genes; others are in regions with few known functions. There is no indication of a possible function for IS200. The method described here should be applicable to the mapping of IS elements in general.
摘要
野生型鼠伤寒沙门氏菌LT2菌株含有六个插入序列元件IS200拷贝，这是沙门氏菌特有的。我们已经确定了LT2菌株中IS200所有六个拷贝的染色体位置。这是通过绘制插入在每个IS200拷贝附近的Tn10元件的位置来完成的。通过Southern杂交检测到这些Tn10插入，其作为具有改变的电泳迁移率的含IS200的限制性片段。这些拷贝位于染色体上间隔相当均匀的位点。一些位于具有许多已知基因的区域；其他的则位于功能已知较少的区域。没有迹象表明IS200具有可能的功能。这里描述的方法一般应适用于IS元件的定位。
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