Hoppe I, Johnston H M, Biek D, Roth J R
Genetics. 1979 May;92(1):17-26. doi: 10.1093/genetics/92.1.17.
The hisG gene is the most operator-proximal structural gene of the histidine operon; it encodes the feedback-inhibitable first enzyme of the biosynthetic pathway. Previously, hisG mutants were mapped into seven intervals defined by the availble deletion mutations having endpoints in the hisG gene. The map has been refined using over 60 new deletion mutants. The new map divides the gene into 40 deletion intervals, which average approximately 30 base pairs in length. The map has been used to analyze the distribution of insertion sites for the transposable element Tn10 and has permitted conclusions on the diistribution of duplication endpoints. The map promises to be useful in analysis of his regulation and, more particularly, in the determination of the possible role of the hisG enzyme in this mechanism.
hisG基因是组氨酸操纵子中最靠近操纵基因的结构基因；它编码生物合成途径中可被反馈抑制的第一种酶。以前，hisG突变体被定位到由hisG基因中具有端点的可用缺失突变所定义的七个区间。利用60多个新的缺失突变体对该图谱进行了完善。新图谱将该基因分为40个缺失区间，平均长度约为30个碱基对。该图谱已用于分析转座因子Tn10插入位点的分布，并得出了重复端点分布的结论。该图谱有望在his调控分析中发挥作用，更具体地说，在确定hisG酶在该机制中可能发挥的作用方面发挥作用。
