鉴定控制Tn10编码的四环素抗性表达的阻遏物结合位点。
Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10.
作者信息
Wray L V, Reznikoff W S
出版信息
J Bacteriol. 1983 Dec;156(3):1188-91. doi: 10.1128/jb.156.3.1188-1191.1983.
Abstract
The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo repressor titration assay. The results indicate that repressor specifically binds both regions of dyad symmetry.
摘要
控制四环素抗性和阻遏基因表达的调控区域包含两个几乎相同的二重对称区域。通过用S1核酸酶消化分离出该控制区域的缺失片段。通过体内阻遏物滴定测定法确定这些缺失片段结合四环素阻遏物的能力。结果表明，阻遏物特异性结合二重对称的两个区域。
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