Tn10编码的四环素抗性基因的核苷酸序列。
Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.
作者信息
Hillen W, Schollmeier K
出版信息
Nucleic Acids Res. 1983 Jan 25;11(2):525-39. doi: 10.1093/nar/11.2.525.
Abstract
The nucleotide sequence of 1530 base pairs of Tn10 DNA coding for tetracycline resistance has been determined. The gene start consists of overlapping bidirectional promotors and two operator sequences. One terminator of transcription as defined by the typical terminator sequence is about 1300 base pairs downstream from the promotor. It is preceeded by translation termination codons in all three possible reading frames. The transcript contains an open reading frame coding for a 43.3 kDa protein. Two other possible reading frames are discussed. The amino acid sequence of the Tn10 encoded tetracycline resistance gene shows good homology with two proteins encoded in the tetracycline resistance part of the plasmid pBR322. The hydrophobic nature of the 43.3 kDa protein is discussed with regard to it's proposed function.
摘要
编码四环素抗性的Tn10 DNA的1530个碱基对的核苷酸序列已被确定。基因起始由重叠的双向启动子和两个操纵序列组成。由典型终止子序列定义的一个转录终止子位于启动子下游约1300个碱基对处。在所有三种可能的阅读框中，它之前都有翻译终止密码子。转录本包含一个编码43.3 kDa蛋白质的开放阅读框。还讨论了另外两个可能的阅读框。Tn10编码的四环素抗性基因的氨基酸序列与质粒pBR322的四环素抗性部分编码的两种蛋白质具有良好的同源性。针对其推测的功能，讨论了43.3 kDa蛋白质的疏水性。
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