Cowman R A, Baron S S, Obenauf S D, Byrnes J J
J Clin Microbiol. 1983 Nov;18(5):1177-82. doi: 10.1128/jcm.18.5.1177-1182.1983.
A procedure was developed for determining the relative levels of lactoperoxidase, leukocyte myeloperoxidase, and thiocyanate-sensitive peroxidase in human saliva. With this procedure, most of the peroxidase activity in whole saliva from normal (those without cancer) subjects was found to be associated with lactoperoxidase and thiocyanate-sensitive peroxidase, with only a minor contribution from leukocyte myeloperoxidase. In contrast, thiocyanate-sensitive peroxidase and leukocyte myeloperoxidase were the major peroxidase activities present in the residual salivary secretion obtainable from two xerostomic patients examined, and these enzymes were present at concentrations much higher than those normally occurring in human saliva. The occurrence of thiocyanate-sensitive peroxidase in saliva has not been previously reported and may represent either an additional peroxidase activity of saliva or a form of lactoperoxidase which is particularly sensitive to inhibition by thiocyanate.
已开发出一种用于测定人唾液中乳过氧化物酶、白细胞髓过氧化物酶和硫氰酸盐敏感过氧化物酶相对水平的方法。通过该方法发现，正常（无癌症）受试者全唾液中的大部分过氧化物酶活性与乳过氧化物酶和硫氰酸盐敏感过氧化物酶有关，白细胞髓过氧化物酶的贡献较小。相比之下，硫氰酸盐敏感过氧化物酶和白细胞髓过氧化物酶是两名口腔干燥症患者可获得的残余唾液分泌中存在的主要过氧化物酶活性，并且这些酶的浓度远高于人唾液中的正常浓度。唾液中硫氰酸盐敏感过氧化物酶的存在此前尚未见报道，可能代表唾液的一种额外过氧化物酶活性，或者是一种对硫氰酸盐抑制特别敏感的乳过氧化物酶形式。
