Altrock B W, Cardiff R D, Puma J P, Lund J K
J Natl Cancer Inst. 1982 Jun;68(6):1037-41.
Murine mammary tumor virus (MuMTV) provirus sequences in the DNA from early-occurring (average age 10 mo) and late occurring (age greater than 20 mo) tumors in BALB/cfC3H mice were analyzed by Eco RI restriction endonuclease mapping procedures. All early tumors were MuMTV antigen-positive mammary adenocarcinomas that contained the 0.92- and 4.0-kilo base (kb) exogenous C3H MuMTV-specific Pst I restriction endonuclease fragments. All but 1 of the late mammary adenocarcinomas had MuMTV antigens detected by peroxidase antiperoxidase staining, and all contained the 0.92- and 4.0-kb exogenous virus Pst I fragments. Three late nonmammary tumors lacked both MuMTV antigens and acquired provirus sequences. Greater numbers of MuMTV sequences were detected in both early and late-arising mammary tumors by Eco RI restriction endonuclease mapping than were detected in tissues from uninfected BALB/c mice. However, neither the number nor the location of MuMTV proviruses correlated with tumor latent period.
采用Eco RI限制性内切酶图谱分析方法，对BALB/cfC3H小鼠早期发生（平均年龄10个月）和晚期发生（年龄大于20个月）肿瘤的DNA中的鼠乳腺肿瘤病毒（MuMTV）前病毒序列进行了分析。所有早期肿瘤均为MuMTV抗原阳性的乳腺腺癌，含有0.92和4.0千碱基（kb）的外源性C3H MuMTV特异性Pst I限制性内切酶片段。除1例晚期乳腺腺癌外，其余所有肿瘤通过过氧化物酶抗过氧化物酶染色均检测到MuMTV抗原，且均含有0.92和4.0 kb的外源性病毒Pst I片段。3例晚期非乳腺肿瘤既缺乏MuMTV抗原，也没有获得的前病毒序列。通过Eco RI限制性内切酶图谱分析，在早期和晚期发生的乳腺肿瘤中检测到的MuMTV序列数量均多于未感染的BALB/c小鼠组织中的序列数量。然而，MuMTV前病毒的数量和位置均与肿瘤潜伏期无关。
