Amenta F, Cavallotti C, De Rossi M, Vatrella F
Naunyn Schmiedebergs Arch Pharmacol. 1983 Sep;324(2):94-8. doi: 10.1007/BF00497013.
The direct histochemical detection of beta-blocker binding sites was studied in sections of rat kidney using an immunohistochemical technique developed in our laboratory. Frozen sections of rat kidney were incubated in a solution of (-)alprenolol, washed, exposed to fluorescent (-)alprenolol antibodies (FAA) and then observed at a fluorescence microscope. Strong fluorescence was found within the wall of renal artery and vein, but primarly in the artery. At the level of blood vessels (-)alprenolol binding sites were located chiefly in the media and in the intima. The renal glomerulus, the loop of Henle and collecting tubules appear to be free of any fluorescence. Consequently they do not have beta-adrenoceptors. On the contrary, the glomerular afferent and afferent arterioles, the cellular elements of the juxtaglomerular apparatus, proximal and distal convoluted tubules, are rich in (-)alprenolol binding sites. At higher magnifications the immunoreactivity appears to be located in the basal membrane of cellular elements which indicates that (-)alprenolol binding sites are membrane receptors. The direct immunohistochemical detection of beta-blocker binding sites in the kidney may offer useful information concerning the site of action of beta-blockers at the level of an important target organ for this class of drugs.
利用我们实验室开发的免疫组织化学技术，对大鼠肾脏切片中β受体阻滞剂结合位点进行了直接组织化学检测。将大鼠肾脏冰冻切片置于（-）阿普洛尔溶液中孵育，冲洗后，用荧光（-）阿普洛尔抗体（FAA）处理，然后在荧光显微镜下观察。在肾动脉和静脉壁内发现强荧光，但主要在动脉中。在血管水平，（-）阿普洛尔结合位点主要位于中膜和内膜。肾小球、亨氏袢和集合小管似乎没有任何荧光。因此，它们没有β肾上腺素能受体。相反，肾小球入球和出球小动脉、球旁器的细胞成分、近端和远端曲管富含（-）阿普洛尔结合位点。在更高放大倍数下，免疫反应性似乎位于细胞成分的基底膜中，这表明（-）阿普洛尔结合位点是膜受体。在肾脏中对β受体阻滞剂结合位点进行直接免疫组织化学检测，可能会提供有关β受体阻滞剂在这类药物的一个重要靶器官水平上的作用位点的有用信息。
