Luiten R G, Schoenmakers J G, Konings R N
Nucleic Acids Res. 1983 Nov 25;11(22):8073-85. doi: 10.1093/nar/11.22.8073.
From in vitro protein synthesis studies and nucleotide sequence analysis it has been deduced that, unlike the major coat proteins of the hitherto studied filamentous bacterial viruses Ff (M13, fd and f1), IKe and Pf1, the major coat protein of the filamentous Pseudomonas aeruginosa virus Pf3 is not synthesized as a precursor containing a leader signal polypeptide at its N-terminal end. From the elucidated nucleotide sequence of the Pf3 major coat protein gene it follows that the coat protein is 44 amino acid residues long (mol.wt. 6425). No sequence homology was observed with the major coat protein genes of either the Ff group or IKe but, similar to these phages, 3' ward of the Pf3 coat protein gene a DNA sequence is located which has many characteristics in common with rho-independent transcription termination signals.
通过体外蛋白质合成研究和核苷酸序列分析推断，与迄今研究的丝状细菌病毒Ff（M13、fd和f1）、IKe和Pf1的主要衣壳蛋白不同，铜绿假单胞菌丝状病毒Pf3的主要衣壳蛋白不是作为在其N末端含有前导信号多肽的前体合成的。从已阐明的Pf3主要衣壳蛋白基因的核苷酸序列可知，衣壳蛋白长44个氨基酸残基（分子量6425）。未观察到与Ff组或IKe的主要衣壳蛋白基因有序列同源性，但与这些噬菌体相似，在Pf3衣壳蛋白基因的3'端有一个DNA序列，它与不依赖ρ的转录终止信号有许多共同特征。
