转座子Tn10末端的必需位点。
Essential sites at transposon Tn 10 termini.
作者信息
Way J C, Kleckner N
出版信息
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3452-6. doi: 10.1073/pnas.81.11.3452.
Abstract
We describe here point and deletion mutations that define which sequences at the termini of Tn10 are essential for transposition. We conclude that at least 13 and no more than 27 base pairs of terminal IS10 sequence are absolutely required at each end. These sequences correspond closely to the terminal inverted repeats of IS10. Sequences between base pairs 27 and 70 at each terminus and certain non-IS10 sequences can also influence transposition, but to a lesser degree. We also describe properties of many function-defective Tn10 transposition mutants and one exceptional Tn10 mutant.
摘要
我们在此描述了点突变和缺失突变，这些突变确定了Tn10末端的哪些序列对于转座是必不可少的。我们得出结论，每一端至少需要13个且不超过27个碱基对的末端IS10序列。这些序列与IS10的末端反向重复序列紧密对应。每个末端碱基对27至70之间的序列以及某些非IS10序列也会影响转座，但程度较小。我们还描述了许多功能缺陷型Tn10转座突变体和一个特殊的Tn10突变体的特性。
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