Magrath I, Erikson J, Whang-Peng J, Sieverts H, Armstrong G, Benjamin D, Triche T, Alabaster O, Croce C M
Science. 1983 Dec 9;222(4628):1094-8. doi: 10.1126/science.6316501.
Three cell lines were derived from a homosexual patient with probable acquired immunodeficiency syndrome and Burkitt's lymphoma. The cell lines produce an unusual strain of Epstein-Barr virus which will both transform cord blood lymphocytes and induce early antigens in Raji cells. Translocations between chromosomes 8 and 22 have occurred in all three lines, but the cells synthesize immunoglobulin M with light chains of the kappa type, in contrast to the usual concordance between a translocation involving chromosome 22 and lambda chain synthesis. Both kappa genes and one lambda gene are rearranged. These findings indicate either that translocation may occur as a separate event from immunoglobulin gene rearrangement or that the proposed hierarchical sequence of immunoglobulin gene rearrangements is not always adhered to. The data also imply that in cells containing a translocation between the long arm of chromosome 8 and a chromosome bearing an immunoglobulin gene, alteration of cellular myc expression may occur regardless of the immunoglobulin gene that is expressed.
从一名患有可能的获得性免疫缺陷综合征和伯基特淋巴瘤的同性恋患者身上获得了三株细胞系。这些细胞系产生了一种不寻常的爱泼斯坦-巴尔病毒株，它既能转化脐血淋巴细胞，又能在拉吉细胞中诱导早期抗原。在所有三株细胞系中均发生了8号和22号染色体之间的易位，但与涉及22号染色体的易位和λ链合成之间通常的一致性相反，这些细胞合成κ型轻链的免疫球蛋白M。κ基因和一个λ基因均发生了重排。这些发现表明，易位可能作为与免疫球蛋白基因重排无关的独立事件发生，或者免疫球蛋白基因重排的提议层级顺序并非总是遵循。数据还暗示，在含有8号染色体长臂与携带免疫球蛋白基因的染色体之间易位的细胞中，无论表达何种免疫球蛋白基因，细胞myc表达可能都会发生改变。
