Haider S G, Urban A, Hilscher B, Hilscher W, Passia D
Andrologia. 1983 Sep-Oct;15(5):498-506. doi: 10.1111/j.1439-0272.1983.tb00176.x.
The influence of cyproterone acetate (CA) upon the behaviour of hydroxysteroid dehydrogenases (HSDH) in the Wistar rat Leydig cells was investigated during the perinatal phase with the help of enzymhistochemical cum morphometrical techniques. The pregnant rats as well as their offsprings were injected with CA (dosage: 35 mg/kg body wt) sc, daily from 14 fetal day upto 31 postnatal day (p.n.d.). The animals were killed on 5, 20, and 32 p.n.d.; the enzymhistochemical reactions for 3-beta-HSDH, 11-beta-HSDH, 17-HSDH and 3-alpha-HSDH were performed in the cryostat sections of the testis, and the morphometric evaluation of HSDH positive Leydig cells was carried out. On 5 p.n.d. the activity of 17-beta-HSDH was slightly impaired in the intertubular Leydig cells of the CA treated animals. On 20 p.n.d., CA prevented nearly completely the HSDH activity in the newsly built peritubular Leydig cells; the activities of 3-beta-HSDH, 17-beta-HSDH, and 3-alpha-HSDH resided mainly in the intertubular Leydig cells. On 32 p.n.d. the HSDH activities in the Leydig cells were observed in the control as well as in treated animals. It seems that the differentiation of peritubular Leydig cells, and thereby the steroid production, is delayed by CA, but not entirely blocked.
在围产期，借助酶组织化学和形态计量学技术，研究了醋酸环丙孕酮（CA）对Wistar大鼠睾丸间质细胞中羟基类固醇脱氢酶（HSDH）行为的影响。从胎儿第14天到出生后第31天（p.n.d.），每天给怀孕大鼠及其后代皮下注射CA（剂量：35 mg/kg体重）。在出生后第5、20和32天处死动物；在睾丸的低温切片上进行3-β-HSDH、11-β-HSDH、17-HSDH和3-α-HSDH的酶组织化学反应，并对HSDH阳性的睾丸间质细胞进行形态计量评估。在出生后第5天，CA处理动物的曲细精管间睾丸间质细胞中17-β-HSDH的活性略有受损。在出生后第20天，CA几乎完全抑制了新形成的曲细精管周围睾丸间质细胞中的HSDH活性；3-β-HSDH、17-β-HSDH和3-α-HSDH的活性主要存在于曲细精管间睾丸间质细胞中。在出生后第32天，在对照组和处理组动物的睾丸间质细胞中均观察到HSDH活性。似乎曲细精管周围睾丸间质细胞的分化以及由此产生的类固醇生成被CA延迟，但并未完全阻断。
