Neumann A, Haider S G, Hilscher B
Department of Anatomy II, Heinrich-Heine-University Düsseldorf, Germany.
Andrologia. 1993 Sep-Oct;25(5):263-9. doi: 10.1111/j.1439-0272.1993.tb02723.x.
The serial semithin sections of the testes of perfused rats (age: 25-41 postnatal days) were evaluated microscopically on each alternative day for the morphology of spermatogenetic cells and especially for spermatid differentiation. The histochemical reaction for non-specific esterases, 3 alpha-hydroxysteroid-dehydrogenase (HSDH), 3 beta-HSDH, 11 beta-HSDH, 17 beta-HSDH were performed in cryostat sections of corresponding rat testes (age: 25-55 days). Eight different types of germ cell associations (designated as A-H) appeared from day 25 to day 41. Seven steps of differentiation of spermatids were characterized. The turning point of spermatid maturation was observed on day 35. Prior to this day, mainly spermatids step 1 were identified. On day 35 spermatids steps 1-5 were present. The reactions of non-specific esterases, 3 beta-HSDH, 17 beta-HSDH and 3 alpha-HSDH were observed in the Leydig cells of all testes. Only from day 35 onwards could 11 beta-HSDH be demonstrated. The temporal sequence of the differentiation of spermatids and the appearance of histochemical activity of 11 beta-HSDH in the Leydig cells seem to be closely correlated. An additional observation concerning the presence of germ cell debris was made in the seminiferous tubules during the postnatal development. Large amounts of debris were observed only till day 33.
对灌注大鼠（出生后25 - 41天）睾丸的系列半薄切片每隔一天进行显微镜检查，以评估生精细胞的形态，尤其是精子细胞的分化。对相应大鼠睾丸（年龄：25 - 55天）的冷冻切片进行非特异性酯酶、3α - 羟基类固醇脱氢酶（HSDH）、3β - HSDH、11β - HSDH、17β - HSDH的组织化学反应。从第25天到第41天出现了八种不同类型的生殖细胞组合（命名为A - H）。确定了精子细胞分化的七个阶段。在第35天观察到精子细胞成熟的转折点。在这一天之前，主要鉴定出第1阶段的精子细胞。在第35天，出现了第1 - 5阶段的精子细胞。在所有睾丸的间质细胞中均观察到非特异性酯酶、3β - HSDH、17β - HSDH和3α - HSDH的反应。仅从第35天起才能检测到11β - HSDH。精子细胞分化的时间顺序与间质细胞中11β - HSDH组织化学活性的出现似乎密切相关。在出生后发育过程中，对生精小管中生殖细胞碎片的存在进行了额外观察。仅在第33天之前观察到大量碎片。
