Merten O W, Dautzenberg H, Palfi G E
Institut Pasteur, Technologie Cellulaire, Paris, France.
Cytotechnology. 1991 Oct;7(2):121-30. doi: 10.1007/BF00350918.
A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammonium chloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process.
开发了一种用于培养哺乳动物细胞的新封装方法。使用硫酸纤维素钠（CS）（1.5%）和聚二甲基二烯丙基氯化铵（PDMDAAC）溶液制备胶囊。当CS液滴落入由2%的PDMDAAC溶液组成的沉淀浴中时，立即在相间形成一层膜。测试了不同封装工艺参数对胶囊特性、细胞生长和单克隆抗体产生的影响。与其他方法相比，这种新方法显示出优势，主要是因为整个过程时间简单。
