Enrietto P J, Payne L N, Hayman M J
Cell. 1983 Dec;35(2 Pt 1):369-79. doi: 10.1016/0092-8674(83)90170-8.
The avian myelocytomatosis virus MC29 induces neoplastic diseases in chicken, including myelocytomas and tumors of kidney and liver, which are due to the action of the v-myc gene. However, MC29 has never been reported to cause lymphoid tumors, the disease associated with activation of the c-myc gene by the insertion of a lymphoid leukosis virus genome. We have analyzed a recovered MC29 virus, HBI, which has a myc gene containing c-myc sequences, acquired by recombination with the cellular gene, and some v-myc sequences. Inoculation of HBI into chickens resulted in lymphoid tumors independent of the bursa. Antigenically these tumors were made up of T and B cells. Molecular analysis showed HBI proviral DNA in 36 of 39 tumors analyzed, with no obvious alteration of c-myc, and the HBI gag-myc fusion protein, p 108, could be detected in tumor cells. These data are discussed in terms of the mechanism of target-cell specificity for transformation by the myc gene.
禽骨髓细胞瘤病毒MC29可在鸡中诱发肿瘤性疾病，包括骨髓细胞瘤以及肾脏和肝脏肿瘤，这些都是由v-myc基因的作用引起的。然而，从未有报道称MC29会引发淋巴肿瘤，这种疾病与淋巴白血病病毒基因组插入导致c-myc基因激活有关。我们分析了一株回收的MC29病毒HBI，它具有一个包含c-myc序列的myc基因，该基因是通过与细胞基因重组获得的，同时还含有一些v-myc序列。将HBI接种到鸡体内会导致独立于法氏囊的淋巴肿瘤。从抗原角度来看，这些肿瘤由T细胞和B细胞组成。分子分析显示，在分析的39个肿瘤中有36个含有HBI前病毒DNA，c-myc没有明显改变，并且在肿瘤细胞中可以检测到HBI gag-myc融合蛋白p 108。本文根据myc基因转化的靶细胞特异性机制对这些数据进行了讨论。
