白蛋白可稳定14,15-白三烯A4。

Albumin stabilizes 14,15-leukotriene A4.

作者信息

Haeggström J, Fitzpatrick F, Rådmark O, Samuelsson B

出版信息

FEBS Lett. 1983 Nov 28;164(1):181-4. doi: 10.1016/0014-5793(83)80045-3.

Abstract

14,15-Leukotriene A4 is a pivotal biosynthetic intermediate in 15-lipoxygenase initiated leukotriene biosynthesis. This compound hydrolyzes instantaneously in phosphate buffer at pH 7.4. However, addition of human or bovine albumin to otherwise identical buffer solutions increases its stability. Intact 14,15-leukotriene A4 then decomposes by first-order kinetics with rate constants inversely proportional to the albumin concentration. Stabilization of 14,15-leukotriene A4 under certain conditions may influence its proportionate transformation by enzymatic vs non-enzymatic processes.

摘要

14,15-白三烯A4是15-脂氧合酶启动的白三烯生物合成中的关键生物合成中间体。该化合物在pH 7.4的磷酸盐缓冲液中会立即水解。然而，在其他条件相同的缓冲溶液中加入人或牛血清白蛋白会增加其稳定性。完整的14,15-白三烯A4随后以一级动力学分解，速率常数与白蛋白浓度成反比。在某些条件下14,15-白三烯A4的稳定化可能会影响其通过酶促与非酶促过程的比例转化。