Yamaguchi N, Kawai K
Gastroenterol Jpn. 1983 Oct;18(5):428-35. doi: 10.1007/BF02776581.
CEA producing cell lines were established from human gastric cancer (HGC-Y1), pancreatic cancer (HPC-Y9) and lung cancer (HLC-Y1). The culture medium was used RPMI-1640 supplemented with 10% fetal bovine serum. The secretion of carcinoembryonic antigen (CEA) into the spent medium from these cultured cell lines was modified by several factors, such as theophylline, cyclic AMP (cAMP), dibutyryl cyclic AMP (dbcAMP), Bromodeoxyuridine (BrdUrd), dimethyl sulfoxide (DMSO), Prostaglandin E2 (PGE2) and human interferon (INF). CEA secretion was enhanced by theophylline, cAMP, PGE2 and INF. Theophylline had an optimal dose to maximally enhance CEA secretion. cAMP and INF apparently enhanced CEA secretion dose dependently. PGE2 appeared to enhance CEA secretion, although cell growth was markedly suppressed dose dependently, dbcAMP, DMSO and BrdUrd did not affect CEA secretion. Here, the kinetics of CEA secretion was discussed.
从人胃癌（HGC-Y1）、胰腺癌（HPC-Y9）和肺癌（HLC-Y1）中建立了产生癌胚抗原（CEA）的细胞系。培养基采用添加10%胎牛血清的RPMI-1640。这些培养的细胞系向用过的培养基中分泌癌胚抗原（CEA）的情况受到多种因素的影响，如茶碱、环磷酸腺苷（cAMP）、二丁酰环磷酸腺苷（dbcAMP）、溴脱氧尿苷（BrdUrd）、二甲基亚砜（DMSO）、前列腺素E2（PGE2）和人干扰素（INF）。茶碱、cAMP、PGE2和INF可增强CEA的分泌。茶碱有一个最大增强CEA分泌的最佳剂量。cAMP和INF明显呈剂量依赖性地增强CEA分泌。尽管细胞生长明显呈剂量依赖性地受到抑制，但PGE2似乎能增强CEA分泌，dbcAMP、DMSO和BrdUrd对CEA分泌没有影响。在此，对CEA分泌的动力学进行了讨论。
