Luka J, Jörnvall H, Klein G
Intervirology. 1983;20(4):213-22. doi: 10.1159/000149394.
The Epstein-Barr virus(EBV)-determined nuclear antigen (EBNA) was purified from a variety of EBV-carrying nonproducer cells. Antigen was detected by Western blotting and subsequent visualization of complexes with antiserum/protein A. All cell lines contained several EBNA components, ranging in molecular weight from 48,000 (48K) to 73K. Even higher-molecular-weight forms of EBNA-positive immunoreactivity were detected, but in much lower yield and in only some preparations (81K in Raji; 120K in Namalva and Raji). Preparations in which the 48K, 65K, 70K, or 73K polypeptides predominated had amino acid compositions with largely similar distributions for most residues. The preparations also yielded similarly sized large fragments upon proteolytic treatments. These findings suggested that all structures are related and that the 48K component may be a part of, or a degradation product from, the larger 65-73K components.
爱泼斯坦-巴尔病毒（EBV）核抗原（EBNA）是从多种携带EBV的非生产性细胞中纯化出来的。通过蛋白质印迹法以及随后用抗血清/蛋白A对复合物进行可视化检测来检测抗原。所有细胞系都含有几种EBNA成分，分子量范围从48,000（48K）到73K。甚至还检测到了分子量更高的EBNA阳性免疫反应形式，但产量低得多，且仅在某些制剂中出现（拉吉细胞系中为81K；纳马勒瓦细胞系和拉吉细胞系中为120K）。以48K、65K、70K或73K多肽为主的制剂，其大多数残基的氨基酸组成分布基本相似。这些制剂在进行蛋白水解处理后也产生了大小相似的大片段。这些发现表明所有结构都相关，并且48K成分可能是较大的65 - 73K成分的一部分或降解产物。
