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人巨细胞病毒一种64,000道尔顿糖蛋白的分离及部分化学特性分析

Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus.

作者信息

Clark B R, Zaia J A, Balce-Directo L, Ting Y P

出版信息

J Virol. 1984 Jan;49(1):279-82. doi: 10.1128/JVI.49.1.279-282.1984.

DOI:10.1128/JVI.49.1.279-282.1984
PMID:6317893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255455/
Abstract

A guanidinium chloride extract of [3H]glucosamine- and [35S]methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.

摘要

用反相高压液相色谱法分离了经[3H]氨基葡萄糖和[35S]甲硫氨酸标记的人巨细胞病毒（汤氏株）病毒体加致密体的氯化胍提取物。对洗脱液进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，主峰是一种相对分子质量为64,000的糖蛋白。通过氨基酸分析及其胰蛋白酶肽段的高压液相色谱图对这种糖蛋白（HCMVgp64）进行了表征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6c/255455/d85e83c70b4a/jvirol00136-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6c/255455/2f5bf0f5795c/jvirol00136-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6c/255455/d85e83c70b4a/jvirol00136-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6c/255455/2f5bf0f5795c/jvirol00136-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6c/255455/d85e83c70b4a/jvirol00136-0296-b.jpg

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本文引用的文献

1
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Infect Immun. 1982 Jun;36(3):933-42. doi: 10.1128/iai.36.3.933-942.1982.
2
Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants.抗人巨细胞病毒单克隆抗体：三种具有独特免疫和电泳特性的表面膜蛋白可确定交叉反应决定簇。
Infect Immun. 1982 Jun;36(3):924-32. doi: 10.1128/iai.36.3.924-932.1982.
3
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
编码人巨细胞病毒64千道尔顿主要晚期抗原的基因片段的克隆与物理图谱绘制。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4965-9. doi: 10.1073/pnas.81.15.4965.
4
Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus.人巨细胞病毒两种病毒体磷蛋白pp65和pp71编码基因的一级结构与转录
J Virol. 1987 Feb;61(2):446-53. doi: 10.1128/JVI.61.2.446-453.1987.
5
Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection.人巨细胞病毒晚期感染期间存在的三种与人巨细胞病毒RNA同源的cDNA克隆的分子克隆与分析。
J Virol. 1986 Nov;60(2):531-8. doi: 10.1128/JVI.60.2.531-538.1986.
6
Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens.用于检测抗人巨细胞病毒诱导膜抗原抗体的免疫荧光法。
J Clin Microbiol. 1986 Sep;24(3):405-13. doi: 10.1128/jcm.24.3.405-413.1986.
7
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8
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9
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10
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Clin Exp Immunol. 1991 Jun;84(3):508-14.
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Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
4
Viscosity-density gradient for purification of foot-and-mouth disease virus.
Arch Gesamte Virusforsch. 1972;36(1):141-6. doi: 10.1007/BF01250304.
5
Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells.抗水痘-带状疱疹病毒诱导膜抗原的抗体：使用单分散戊二醛固定靶细胞的免疫荧光测定法
J Infect Dis. 1977 Oct;136(4):519-30. doi: 10.1093/infdis/136.4.519.
6
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J Virol. 1976 Dec;20(3):604-11. doi: 10.1128/JVI.20.3.604-611.1976.
7
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8
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9
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10
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