Zaia J A, Oxman M N
J Infect Dis. 1977 Oct;136(4):519-30. doi: 10.1093/infdis/136.4.519.
A sensitive and reproducible technique was developed for the detection of antibody to varicella-zoster virus-induced membrane antigen (VZMA) by immunofluorescence. Controlled trypsinization and glutaraldehyde fixation were employed to prepare a monodisperse suspension of noninfectious VZMA-positive target cells that can be stored indefinitely at -72 degrees C. A microtiter immunofluorescence assay utilizing these target cells was shown to provide a sensitive and specific means for the detection and quantitation of antibody to varicella-zoster virus. The properties of the target cell preparation and the characteristics of the assay make practical the rapid assessment of immunity to the varicella-zoster virus.
已开发出一种灵敏且可重复的技术，用于通过免疫荧光检测水痘 - 带状疱疹病毒诱导的膜抗原（VZMA）抗体。采用可控胰蛋白酶消化和戊二醛固定来制备非感染性VZMA阳性靶细胞的单分散悬浮液，该悬浮液可在 -72℃无限期保存。利用这些靶细胞的微量滴定免疫荧光测定法被证明为检测和定量水痘 - 带状疱疹病毒抗体提供了一种灵敏且特异的方法。靶细胞制剂的特性和测定法的特点使得快速评估对水痘 - 带状疱疹病毒的免疫力成为可能。
