Costa R H, Cohen G, Eisenberg R, Long D, Wagner E
J Virol. 1984 Jan;49(1):287-92. doi: 10.1128/JVI.49.1.287-292.1984.
The two partially colinear 6-kilobase (kb) and 1.5-kb mRNAs mapping between 0.23 and 0.27 map units on the herpes simplex virus type 1 genome were precisely located. The 5' end of the 6-kb mRNA was located 28 bases downstream of the sequence ATATATT and was 10 bases to the left of the BamHI site at 0.268. This position is ca. 90 bases to the left of our earlier reported sequence (R. J. Frink, K. G. Draper, and E. K. Wagner, Proc. Natl. Acad. Sci. U.S.A. 78:6139-6143, 1981). We used a polyclonal antibody made against purified herpes simplex virus type 1 VP5 to demonstrate that the 155,000-dalton translation product of the 6-kb mRNA is this capsid protein. The antibody did not react with the 35,000-dalton translation product of the 1.5-kb mRNA. We also confirmed our identification of VP5 as the translation product of the 6-kb mRNA by comparison of tryptic peptides of the in vitro-translated protein and authentic VP5.
在单纯疱疹病毒1型基因组上，定位在0.23至0.27个图距单位之间的两个部分共线的6千碱基（kb）和1.5 kb的信使核糖核酸（mRNA）被精确地定位。6 kb mRNA的5'端位于序列ATATATT下游28个碱基处，且在0.268处BamHI位点左侧10个碱基处。该位置大约在我们之前报道的序列（R. J. Frink、K. G. Draper和E. K. Wagner，《美国国家科学院院刊》78:6139 - 6143，1981年）左侧90个碱基处。我们使用针对纯化的单纯疱疹病毒1型VP5制备的多克隆抗体来证明6 kb mRNA的155,000道尔顿翻译产物就是这种衣壳蛋白。该抗体不与1.5 kb mRNA的35,000道尔顿翻译产物发生反应。我们还通过比较体外翻译蛋白和天然VP5的胰蛋白酶肽段，证实了我们将VP5鉴定为6 kb mRNA的翻译产物。
