通过显微注射克隆的互补脱氧核糖核酸到真核细胞核中来表达病毒膜蛋白。 - Suppr

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超能文献

Expression of viral membrane proteins from cloned cDNA by microinjection into eukaryotic cell nuclei.

作者信息

Timm B, Kondor-Koch C, Lehrach H, Riedel H, Edström J E, Garoff H

出版信息

Methods Enzymol. 1983;96:496-511. doi: 10.1016/s0076-6879(83)96043-3.

DOI:10.1016/s0076-6879(83)96043-3

PMID:6318022

Abstract

摘要

相似文献

Expression of viral membrane proteins from cloned cDNA by microinjection into eukaryotic cell nuclei.通过显微注射克隆的互补脱氧核糖核酸到真核细胞核中来表达病毒膜蛋白。

Methods Enzymol. 1983;96:496-511. doi: 10.1016/s0076-6879(83)96043-3.

Expression of the structural proteins of Semliki Forest virus from cloned cDNA microinjected into the nucleus of baby hamster kidney cells.将克隆的互补脱氧核糖核酸显微注射到幼仓鼠肾细胞核中后，塞姆利基森林病毒结构蛋白的表达

Proc Natl Acad Sci U S A. 1982 Aug;79(15):4525-9. doi: 10.1073/pnas.79.15.4525.

Modified semliki forest virus expression vector that facilitates cloning.便于克隆的改良型塞姆利基森林病毒表达载体。

Biotechniques. 1997 Sep;23(3):432-4, 436. doi: 10.2144/97233bm18.

Expression of Semliki Forest virus E1 protein in Escherichia coli. Low pH-induced pore formation.辛德毕斯病毒E1蛋白在大肠杆菌中的表达。低pH诱导的孔形成。

J Biol Chem. 2001 May 4;276(18):15453-7. doi: 10.1074/jbc.M011061200. Epub 2001 Feb 13.

Expression of Semliki Forest virus capsid protein from SV40 recombinant virus.来自SV40重组病毒的Semliki森林病毒衣壳蛋白的表达。

FEBS Lett. 1985 Jul 1;186(1):59-64. doi: 10.1016/0014-5793(85)81339-9.

Complete nucleotide sequence of the nonstructural protein genes of Semliki Forest virus.Semliki森林病毒非结构蛋白基因的完整核苷酸序列

Nucleic Acids Res. 1986 Jul 25;14(14):5667-82. doi: 10.1093/nar/14.14.5667.

Structural complexity of defective-interfering RNAs of Semliki Forest virus as revealed by analysis of complementary DNA.通过互补DNA分析揭示的Semliki森林病毒缺陷干扰RNA的结构复杂性

Nucleic Acids Res. 1981 Jul 24;9(14):3403-17. doi: 10.1093/nar/9.14.3403.

Semliki Forest virus vectors for large-scale production of recombinant proteins.

Methods Mol Med. 2003;76:525-43. doi: 10.1385/1-59259-304-6:525.

Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.编码Semliki森林病毒膜糖蛋白的cDNA的核苷酸序列。

Nature. 1980 Nov 20;288(5788):236-41. doi: 10.1038/288236a0.

Semliki Forest virus: a probe for membrane traffic in the animal cell.Semliki森林病毒：动物细胞中膜转运的一种探针。

Adv Protein Chem. 1984;36:79-132. doi: 10.1016/s0065-3233(08)60296-x.

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Expression of Semliki Forest virus proteins from cloned complementary DNA. II. The membrane-spanning glycoprotein E2 is transported to the cell surface without its normal cytoplasmic domain.从克隆的互补DNA表达塞姆利基森林病毒蛋白。II. 跨膜糖蛋白E2在没有其正常胞质结构域的情况下被转运到细胞表面。

J Cell Biol. 1983 Sep;97(3):652-8. doi: 10.1083/jcb.97.3.652.

Expression of Semliki Forest virus proteins from cloned complementary DNA. I. The fusion activity of the spike glycoprotein.从克隆的互补DNA表达Semliki森林病毒蛋白。I. 刺突糖蛋白的融合活性。

J Cell Biol. 1983 Sep;97(3):644-51. doi: 10.1083/jcb.97.3.644.

Regulated expression of genes injected into early Drosophila embryos.注入早期果蝇胚胎的基因的调控表达。

EMBO J. 1984 Jan;3(1):165-73. doi: 10.1002/j.1460-2075.1984.tb01778.x.

Microinjection of mRNA coding for an anti-Golgi antibody inhibits intracellular transport of a viral membrane protein.显微注射编码抗高尔基体抗体的信使核糖核酸会抑制病毒膜蛋白的细胞内运输。

Cell. 1984 Apr;36(4):847-56. doi: 10.1016/0092-8674(84)90034-5.

Microinjection of somatic cells with micropipettes: comparison with other transfer techniques.用微量移液器对体细胞进行显微注射：与其他转移技术的比较。

Biochem J. 1984 Oct 15;223(2):281-91. doi: 10.1042/bj2230281.

Cell surface expression of fusogenic vesicular stomatitis virus G protein from cloned cDNA.来自克隆cDNA的融合性水泡性口炎病毒G蛋白的细胞表面表达。

EMBO J. 1984 Jul;3(7):1477-83. doi: 10.1002/j.1460-2075.1984.tb01999.x.

Mutants of the membrane-binding region of Semliki Forest virus E2 protein. II. Topology and membrane binding.塞姆利基森林病毒E2蛋白膜结合区域的突变体。II. 拓扑结构与膜结合

J Cell Biol. 1986 Mar;102(3):902-10. doi: 10.1083/jcb.102.3.902.

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