用微量移液器对体细胞进行显微注射：与其他转移技术的比较。 - Suppr

Suppr

超能文献

Microinjection of somatic cells with micropipettes: comparison with other transfer techniques.

作者信息

Celis J E

出版信息

Biochem J. 1984 Oct 15;223(2):281-91. doi: 10.1042/bj2230281.

DOI:10.1042/bj2230281

PMID:6093771

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1144298/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8809/1144298/e9ccb9cc1a60/biochemj00317-0011-a.jpg

相似文献

Microinjection of somatic cells with micropipettes: comparison with other transfer techniques.用微量移液器对体细胞进行显微注射：与其他转移技术的比较。

Biochem J. 1984 Oct 15;223(2):281-91. doi: 10.1042/bj2230281.

A new method of microinjection using erythrocyte ghosts and Sendai virus (HVJ).

Nihon Ketsueki Gakkai Zasshi. 1978 Dec;41(6):1041-5.

Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: a review of methods and applications.

Biosci Rep. 1984 Jun;4(6):451-66. doi: 10.1007/BF01122221.

Erythrocyte ghost-mediated microinjection into cells in monolayer cultures: a highly efficient and low toxic technique.

Cell Biol Int Rep. 1987 Jul;11(7):515-24. doi: 10.1016/0309-1651(87)90013-0.

Chemically facilitated microinjection of proteins into intact monolayers of tissue culture cells.化学辅助将蛋白质显微注射到组织培养细胞的完整单层中。

Somatic Cell Genet. 1977 Nov;3(6):603-10. doi: 10.1007/BF01539068.

Optimization of transfection.转染的优化

Curr Protoc Neurosci. 2001 May;Appendix 1:Appendix 1B. doi: 10.1002/0471142301.nsa01bs01.

Influence of dextran sulfate on the fusion of Sendai virus with liposomes.硫酸葡聚糖对仙台病毒与脂质体融合的影响。

Biomed Biochim Acta. 1990;49(7):633-5.

Microinjection of macromolecules into normal murine lymphocytes by cell fusion technique. I. Quantitative microinjection of antibodies into normal splenic lymphocytes.通过细胞融合技术将大分子显微注射到正常小鼠淋巴细胞中。I. 将抗体定量显微注射到正常脾淋巴细胞中。

J Immunol. 1982 Mar;128(3):1090-6.

Microinjection of macromolecules into cultured cells by erythrocyte ghost-cell fusion.通过红细胞空壳-细胞融合将大分子显微注射到培养细胞中。

Methods Enzymol. 1993;221:306-17. doi: 10.1016/0076-6879(93)21026-5.

Red cell-mediated microinjection.

Methods Enzymol. 1987;149:293-300. doi: 10.1016/0076-6879(87)49067-8.

引用本文的文献

Biological Interfaces, Modulation, and Sensing with Inorganic Nano-Bioelectronic Materials.无机纳米生物电子材料的生物界面、调制与传感

Small Methods. 2020 May 11;4(5). doi: 10.1002/smtd.201900868. Epub 2020 Mar 8.

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells.将珠粒载入贴壁培养的人类细胞中的蛋白质和核酸。

J Vis Exp. 2021 Jun 1(172). doi: 10.3791/62559.

Single cell assay with an automated capillary microinjection system.单细胞分析与自动化毛细管微量注射系统。

Cytotechnology. 1991 Feb;5(Suppl 1):93-8. doi: 10.1007/BF00736821.

Biofunctionalized nanoneedles for the direct and site-selective delivery of probes into living cells.用于将探针直接且位点选择性地递送至活细胞的生物功能化纳米针。

Biochim Biophys Acta. 2011 Mar;1810(3):330-8. doi: 10.1016/j.bbagen.2010.05.005. Epub 2010 May 24.

Permeabilizing soybean protoplasts to macromolecules using electroporation and hypotonic shock.采用电穿孔和低渗休克法使大豆原生质体通透大分子。

Plant Physiol. 1987 Jan;83(1):24-8. doi: 10.1104/pp.83.1.24.

Gene transfer in higher animals: theoretical considerations and key concepts.高等动物中的基因转移：理论思考与关键概念

J Biotechnol. 2002 Oct 9;99(1):1-22. doi: 10.1016/s0168-1656(02)00105-0.

The many ways to cross the plasma membrane.穿越质膜的多种方式。

Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4295-8. doi: 10.1073/pnas.081065198. Epub 2001 Mar 27.

Calcium-regulated exocytosis is required for cell membrane resealing.细胞膜重封需要钙调节的胞吐作用。

J Cell Biol. 1995 Dec;131(6 Pt 2):1747-58. doi: 10.1083/jcb.131.6.1747.

Transient correction of genetic defects in cultured animal cells by introduction of functional proteins.通过引入功能蛋白对培养的动物细胞中的基因缺陷进行瞬时校正。

Mol Cell Biol. 1987 Aug;7(8):3012-7. doi: 10.1128/mcb.7.8.3012-3017.1987.

Transfection of mammalian cells with plasmid DNA by scrape loading and sonication loading.通过刮擦加载和超声加载用质粒DNA转染哺乳动物细胞。

Proc Natl Acad Sci U S A. 1987 Dec;84(23):8463-7. doi: 10.1073/pnas.84.23.8463.

本文引用的文献

Altering genotype and phenotype by DNA-mediated gene transfer.通过DNA介导的基因转移改变基因型和表型。

Science. 1980 Sep 19;209(4463):1414-22. doi: 10.1126/science.7414320.

Insertion of fluorescent phospholipids into the plasma membrane of a mammalian cell.将荧光磷脂插入哺乳动物细胞的质膜。

J Biol Chem. 1980 Jun 10;255(11):5404-10.

Direct microinjection of rabbit globin mRNA into mouse 3T3 cells. Analysis of the polypeptides synthesized in vivo.将兔珠蛋白mRNA直接显微注射到小鼠3T3细胞中。对体内合成的多肽进行分析。

Exp Cell Res. 1980 Apr;126(2):481-5. doi: 10.1016/0014-4827(80)90293-1.

Microinjection of mRNA and other macromolecules into living cells.将信使核糖核酸和其他大分子显微注射到活细胞中。

Methods Enzymol. 1981;79(Pt B):76-88. doi: 10.1016/s0076-6879(81)79016-5.

Immunospecific targeting of liposomes to cells: a novel and efficient method for covalent attachment of Fab' fragments via disulfide bonds.脂质体对细胞的免疫特异性靶向：一种通过二硫键共价连接Fab'片段的新颖且高效的方法。

Biochemistry. 1981 Jul 7;20(14):4229-38. doi: 10.1021/bi00517a043.

The selective degradation of injected proteins occurs principally in the cytosol rather than in lysosomes.注入蛋白质的选择性降解主要发生在细胞质中，而非溶酶体中。

Cell. 1981 Jul;25(1):83-93. doi: 10.1016/0092-8674(81)90233-6.

Coexistence of three major isoactins in a single sarcoma 180 cell.单个肉瘤180细胞中三种主要同工肌动蛋白的共存。

Cell. 1981 Jul;25(1):195-202. doi: 10.1016/0092-8674(81)90244-0.

Mechanisms of DNA entry into mammalian cells. II. Phagocytosis of calcium phosphate DNA co-precipitate visualized by electron microscopy.DNA进入哺乳动物细胞的机制。II. 电子显微镜观察磷酸钙DNA共沉淀物的吞噬作用。

Exp Cell Res. 1982 May;139(1):223-34. doi: 10.1016/0014-4827(82)90336-6.

Liposomes as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene.

Science. 1982 Jan 8;215(4529):166-8. doi: 10.1126/science.7053567.

Irreversible coupling of immunoglobulin fragments to preformed vesicles. An improved method for liposome targeting.免疫球蛋白片段与预制囊泡的不可逆偶联。一种改进的脂质体靶向方法。

J Biol Chem. 1982 Jan 10;257(1):286-8.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验