Rehmat S, Shapiro J A
Mol Gen Genet. 1983;192(3):416-23. doi: 10.1007/BF00392184.
D3112 is a temperate bacteriophage of P. aeruginosa with heterogeneous sequences at one extremity of the virion DNA molecule. Infection of strain PAOl with phage D3112 results in a 40- to 65-fold increase in the frequency of ami mutants resistant to fluoroacetamide. Nine ami::D3112 prophages have been mapped to distinct sites within the ami locus by Southern blotting experiments with a cloned ami+ probe. All prophages have the same restriction map as the D3112 genome extracted from phage particles. The position of D3112 insertions correlates with the phenotype and reversion behavior of the ami mutants. Induction of D3112cts prophages results in amplification of internal prophage segments as discrete restriction fragments before the terminal viral fragments are visible as sharp hybridizing species. This indicates that D3112 replication is accompanied by recombination of prophage termini to numerous sites in the bacterial genome. Chromosomal junction fragments of an ami::D3112cts prophage are maintained through most of the replication cycle but are cleaved shortly before cell lysis, apparently by the viral encapsidation system.
D3112是铜绿假单胞菌的一种温和噬菌体，在病毒粒子DNA分子的一端具有异质序列。用噬菌体D3112感染PAO1菌株会导致对氟乙酰胺有抗性的ami突变体频率增加40至65倍。通过使用克隆的ami⁺探针进行Southern印迹实验，已将九个ami::D3112原噬菌体定位到ami基因座内的不同位点。所有原噬菌体都具有与从噬菌体颗粒中提取的D3112基因组相同的限制性图谱。D3112插入的位置与ami突变体的表型和回复行为相关。诱导D3112cts原噬菌体导致内部原噬菌体片段作为离散的限制性片段扩增，然后末端病毒片段才可见为清晰的杂交条带。这表明D3112复制伴随着原噬菌体末端与细菌基因组中多个位点的重组。ami::D3112cts原噬菌体的染色体连接片段在大部分复制周期中都得以保留，但在细胞裂解前不久被切割，显然是由病毒包装系统完成的。
