Pravtcheva D D, Ruddle F H, Ellis R W, Scolnick E M
Somatic Cell Genet. 1983 Nov;9(6):681-6. doi: 10.1007/BF01539473.
Mouse-Chinese hamster somatic cell hybrids containing various combinations of mouse chromosomes were analyzed for the presence of the mouse c-Ha-ras (1) sequences after restriction endonuclease digestion and hybridization with a 32P-labeled Ha-ras specific probe according to the procedure of Southern (2). The presence of the mouse c-Ha-ras containing fragment was correlated with the presence of mouse chromosome 7 in the hybrids.
根据Southern(2)的方法,对含有不同小鼠染色体组合的小鼠-中国仓鼠体细胞杂种进行限制性内切酶消化,并用32P标记的Ha-ras特异性探针进行杂交,分析小鼠c-Ha-ras(1)序列的存在情况。杂种中含有小鼠c-Ha-ras的片段的存在与小鼠染色体7的存在相关。