中国仓鼠中RAS原癌基因的定位：对哺乳动物基因连锁保守性和肿瘤形成的影响。

Assignment of RAS proto-oncogenes in Chinese hamsters: implications for mammalian gene linkage conservation and neoplasia.

作者信息

Stallings R L, Crawford B D, Black R J, Chang E H

出版信息

Cytogenet Cell Genet. 1986;43(1-2):2-5. doi: 10.1159/000132289.

Abstract

HRAS and KRAS are the cellular homologs of the oncogenic transforming genes found in the Harvey strain of murine sarcoma virus and the Kirsten murine sarcoma virus, respectively. Phyla as diverse as insects, birds, and mammals possess distinct HRAS and KRAS sequences, suggesting that these genes are essential to metazoa. In this report, we used a clone panel of Chinese hamster X mouse C11D somatic cell hybrids segregating hamster chromosomes to map those genes. Southern filter hybridization analyses of the hybrids revealed that hamster HRAS and KRAS gene sequences are on chromosomes 3 and 8, respectively. These gene assignments are consistent with the conservation of autosomal gene linkage groups observed among hamsters, humans, and mice and may provide insight into specific chromosomal alterations that have been observed during the spontaneous neoplastic transformation of Chinese hamster fibroblasts in vitro.

摘要

HRAS和KRAS分别是在哈维株小鼠肉瘤病毒和柯斯顿小鼠肉瘤病毒中发现的致癌转化基因的细胞同源物。昆虫、鸟类和哺乳动物等不同门类都拥有独特的HRAS和KRAS序列，这表明这些基因对后生动物至关重要。在本报告中，我们使用了一组中国仓鼠X小鼠C11D体细胞杂种克隆面板，这些杂种克隆正在分离仓鼠染色体，以绘制这些基因的图谱。对这些杂种克隆的Southern印迹杂交分析表明，仓鼠HRAS和KRAS基因序列分别位于3号和8号染色体上。这些基因定位与在仓鼠、人类和小鼠中观察到的常染色体基因连锁群的保守性一致，并且可能为体外观察到的中国仓鼠成纤维细胞自发肿瘤转化过程中的特定染色体改变提供见解。