小鼠N-ras的分子克隆与染色体定位

Ryan J, Hart C P, Ruddle F H

Nucleic Acids Res. 1984 Aug 10;12(15):6063-72. doi: 10.1093/nar/12.15.6063.

The murine N-ras gene was cloned by screening an EMBL-3 recombinant phage library with a human N-ras specific probe. Hybridization of two separate unique sequence N-ras probes, isolated from the 5' and 3' flanking sequences of the murine gene, to a mouse-Chinese hamster hybrid mapping panel assigns the N-ras locus to mouse chromosome three.

通过用人N-ras特异性探针筛选EMBL-3重组噬菌体文库克隆了小鼠N-ras基因。从鼠基因的5'和3'侧翼序列分离出的两个单独的独特序列N-ras探针，与小鼠-中国仓鼠杂种定位板杂交，将N-ras基因座定位于小鼠3号染色体。