Steele M K, Gallo R V, Ganong W F
Am J Physiol. 1983 Dec;245(6):R805-10. doi: 10.1152/ajpregu.1983.245.6.R805.
To investigate the possibility that angiotensin II (ANG II) is involved in the regulation of luteinizing hormone (LH) secretion, ANG II was injected intraventricular ANG II caused an increase in plasma LH concentrations in 10 min, whereas intravenous ANG II in the same dose had no significant effect. Intraventricular administration of the ANG II antagonist, saralasin, during the afternoon of proestrus induced a significant decrease in the number of rats ovulating and abolished the ovulatory surge in LH secretion seen in saline-injected control animals. Intravenous saralasin in two different doses failed to inhibit ovulation and produced only a small decrease in the LH surge. In animals treated with intraventricular saralasin, the increase in plasma LH concentration produced by intravenous injection of 50 ng of LH-releasing hormone (LHRH) was normal, indicating no alteration in the sensitivity of the gonadotrops to LHRH. Intraventricular administration of the converting-enzyme inhibitor, enalapril diacid, inhibited ovulation and the ovulatory surge, whereas systemically administered enalapril diacid had no effect on LH secretion. The data suggest that ANG II generated in the brain may play a significant role in the regulation of LH secretion on the day of proestrus.
为研究血管紧张素II（ANG II）是否参与促黄体生成素（LH）分泌的调节，将ANG II脑室内注射。ANG II脑室内注射10分钟后可使血浆LH浓度升高，而相同剂量静脉注射ANG II则无显著作用。在动情前期下午脑室内注射ANG II拮抗剂沙拉新，可使排卵大鼠数量显著减少，并消除生理盐水注射对照动物中出现的LH分泌排卵峰。两种不同剂量静脉注射沙拉新均未能抑制排卵，仅使LH峰略有降低。在脑室内注射沙拉新的动物中，静脉注射50 ng促黄体生成素释放激素（LHRH）所引起的血浆LH浓度升高正常，表明促性腺激素对LHRH的敏感性未发生改变。脑室内注射转化酶抑制剂依那普利二酸可抑制排卵和排卵峰，而全身给药依那普利二酸对LH分泌无影响。这些数据表明，脑内生成的ANG II可能在动情前期当天LH分泌的调节中起重要作用。
