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人淋巴细胞培养中第一次分裂中期的出现情况。

Occurrence of 1st division metaphases in human lymphocyte cultures.

作者信息

Crossen P E, Morgan W F

出版信息

Hum Genet. 1978 Feb 23;41(1):97-100. doi: 10.1007/BF00278875.

DOI:10.1007/BF00278875
PMID:631864
Abstract

Human lymphocytes were cultured for 40, 42 and 44 h and analysed for the number of 1st and 2nd division metaphases using the Brd U-Giemsa technique. The most suitable time to obtain the majority of cells in their 1st division concommittant with an adequate mitotic index was 42 h. It is recommended that laboratories use sister chromatid differential staining to determine the most suitable culture time for the analysis of 1st division metaphases. This will permit accurate comparisons between individuals and laboratories to be made.

摘要

将人类淋巴细胞培养40、42和44小时，使用Brd U-吉姆萨技术分析第一次和第二次分裂中期的细胞数量。获得大多数处于第一次分裂且有足够有丝分裂指数的细胞的最合适时间是42小时。建议实验室使用姐妹染色单体差异染色来确定分析第一次分裂中期最合适的培养时间。这将使得能够在个体和实验室之间进行准确的比较。

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1
Occurrence of 1st division metaphases in human lymphocyte cultures.人淋巴细胞培养中第一次分裂中期的出现情况。
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2
Lymphocyte proliferation in Down's syndrome measured by sister chromatid differential staining.采用姐妹染色单体差别染色法检测唐氏综合征患者的淋巴细胞增殖情况。
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3
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4
Proliferation of PPD-stimulated lymphocytes measured by sister chromatoid differential staining.通过姐妹染色单体差异染色法测定PPD刺激淋巴细胞的增殖情况。
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[Functioning of the nucleolus-organizer regions of the chromosomes of cultured human blood lymphocytes].[培养的人血淋巴细胞染色体核仁组织区的功能]
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Analyses of sister-chromatid exchange and cell-cycle kinetics in mouse T- and B-lymphocytes from peripheral blood cultures.外周血培养的小鼠T淋巴细胞和B淋巴细胞中姐妹染色单体交换及细胞周期动力学分析。
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本文引用的文献

1
Induced chromosomal aberrations in man.人类诱导染色体畸变
Adv Hum Genet. 1972;30(0):99-172. doi: 10.1007/978-1-4757-4429-3_3.
2
Giemsa technique for the detection of sister chromatid exchanges.用于检测姐妹染色单体交换的吉姆萨技术。
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3
Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes.光脉冲对5-溴脱氧尿苷和5-碘脱氧尿苷取代的中国仓鼠染色体诱导的姐妹染色单体交换
与细胞动力学相关的诱导性和自发性染色体畸变的频率及类型
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Giemsa banding patterns of human chromosomes.人类染色体的吉姆萨带型
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5
Chromosome aberrations in lymphocytes after occupational exposure to lead and cadmium.职业性接触铅和镉后淋巴细胞中的染色体畸变
Mutat Res. 1976 Jan;40(1):57-62. doi: 10.1016/0165-1218(76)90023-9.
6
The human leukocyte test system. V. DNA synthesis and mitoses in PHA-stimulated 3-day cultures.人类白细胞检测系统。V. 植物血凝素刺激的3天培养物中的DNA合成与有丝分裂
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Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests.用于诱变试验中染色体畸变分析的人外周血淋巴细胞。
Mutat Res. 1975 Jun;31(3):135-48. doi: 10.1016/0165-1161(75)90082-5.
8
Chromosomal radiosensitivity and karyotype in mice using cultured peripheral blood lymphocytes, and comparison with this system in man.利用培养的外周血淋巴细胞研究小鼠的染色体放射敏感性和核型，并与人类的该系统进行比较。
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Analysis of human lymphocyte cell cycle time in culture measured by sister chromatid differential staining.
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