冻干组织中ESR信号的性质及其与恶性肿瘤的相关性。
The nature of the ESR signal in lyophilized tissue and its relevance to malignancy.
作者信息
Dodd N J, Swartz H M
出版信息
Br J Cancer. 1984 Jan;49(1):65-71. doi: 10.1038/bjc.1984.10.
Comparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.
对从冷冻和冻干组织获得的9GHz和35GHz光谱与含有抗坏血酸的模型系统的光谱进行比较，证实组织“冻干信号”的主要成分是抗坏血酸自由基，它通过吸附在惰性基质上而得以稳定。结果表明，在缺氧条件下该信号的大小是细胞损伤的一种度量，细胞损伤会使细胞内抗坏血酸被氧化。将冻干样品暴露于空气中时，由于可用的组织抗坏血酸发生自氧化，信号会增强。在潮湿的大气条件下，抗坏血酸自由基很容易衰变，留下其他自由基，这些自由基似乎是由抗坏血酸或抗坏血酸自由基与某些组织成分相互作用形成的。结果表明，尽管冻干组织的自由基电子自旋共振信号已得到广泛研究，但它并非肿瘤所特有，与恶性肿瘤也无关。
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