Freijd A, Rosén A
Clin Exp Immunol. 1984 Jan;55(1):204-10.
B cell enriched lymphocytes from different lymphoid organs were compared regarding their ability to produce pneumococcal polysaccharide (PPS) antibodies in vitro after transformation with Epstein-Barr virus. Tonsillar, adenoidal and peripheral blood lymphocytes (PBL) were studied. Fifty thousand B enriched lymphocytes from each organ sample were cultured in each of 88 wells of a microplate. After 30 days the supernatant of each culture was assayed for pneumococcal antibody activity and total IgG and IgM, using enzyme immunosorbent assay. Sera from the organ donors were analysed as well. In the supernatants, significant specific antibody activity was detected only of the IgM class, although IgG production was recorded. The IgM anti-PPS activity of PBL was significantly higher than that of the adenoidal lymphocytes (P less than 0.01), with tonsillar lymphocytes in an intermediate position, although the level of total IgM was of the same magnitude. Sera from children showed the highest IgM anti-PPS activity and at the same time the lowest IgG activity. The opposite relation was recorded in sera from the adult donors. Irrespective of the explanation for different distribution of specifically reactive cells in different lymphoid organs our data indicate distinct compartmentalization of immunocompetent B cell clones.
对来自不同淋巴器官的富含B细胞的淋巴细胞进行了比较，观察它们在经爱泼斯坦-巴尔病毒转化后体外产生肺炎球菌多糖（PPS）抗体的能力。研究了扁桃体、腺样体和外周血淋巴细胞（PBL）。将来自每个器官样本的50000个富含B细胞的淋巴细胞接种到微孔板的88个孔中进行培养。30天后，使用酶联免疫吸附测定法检测每个培养物上清液中的肺炎球菌抗体活性以及总IgG和IgM。还对器官供体的血清进行了分析。在上清液中，虽然记录到了IgG的产生，但仅检测到了显著的IgM类特异性抗体活性。PBL的IgM抗PPS活性显著高于腺样体淋巴细胞（P<0.01），扁桃体淋巴细胞处于中间位置，尽管总IgM水平相当。儿童血清显示出最高的IgM抗PPS活性，同时IgG活性最低。在成人供体的血清中记录到相反的关系。无论对不同淋巴器官中特异性反应性细胞的不同分布作何解释，我们的数据都表明免疫活性B细胞克隆存在明显的区室化。
