Hanna M H, Nowicki J J, Fatone M A
J Bacteriol. 1984 Feb;157(2):345-9. doi: 10.1128/jb.157.2.345-349.1984.
Cyclic AMP was synthesized by Polysphondylium violaceum after starvation and during the preaggregation stage of development. Most of the newly synthesized cyclic AMP accumulated in the extracellular medium, with very little change in the intracellular cyclic AMP concentration. The addition of 10(-3) to 10(-6) M exogenous cyclic AMP to starved amoebae caused a 20 to 50% decrease in the number of aggregation centers formed compared with untreated controls. An aggregation-defective mutant of P. violaceum (strain aggA586) excreted or accumulated very little cyclic AMP. Strain aggA586 aggregated normally in the presence of a dialyzable, excreted product (D factor) produced by wild-type amoebae. When the mutant was incubated with D factor, cyclic AMP accumulated in the medium, and the amount accumulated depended on the amount of D factor added to the mutant amoebae.
饥饿后以及在发育的预聚集阶段，紫多头绒泡菌合成了环磷酸腺苷（cAMP）。大部分新合成的cAMP积累在细胞外培养基中，细胞内cAMP浓度变化很小。与未处理的对照相比，向饥饿的变形虫中添加10⁻³至10⁻⁶M的外源性cAMP会使形成的聚集中心数量减少20%至50%。紫多头绒泡菌的一个聚集缺陷突变体（aggA586菌株）排泄或积累的cAMP非常少。aggA586菌株在存在由野生型变形虫产生的可透析排泄产物（D因子）的情况下能正常聚集。当该突变体与D因子一起孵育时，cAMP在培养基中积累，积累的量取决于添加到突变体变形虫中的D因子的量。
