一株缺乏胸苷酸激酶活性的大肠杆菌突变体的分离与鉴定。
Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activity.
作者信息
Daws T D, Fuchs J A
出版信息
J Bacteriol. 1984 Feb;157(2):440-4. doi: 10.1128/jb.157.2.440-444.1984.
Abstract
Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD105, produced a dTMP kinase with 25-fold lower specific activity and a 5-fold higher Km for dTMP than the parental strain. The dTMP pool in TD105 was 4.4-fold higher than in the parent. In addition to temperature sensitivity and resistance to 2',3'-dideoxythymidine, the mutant exhibited a hypersensitivity to 5-bromo-2'-deoxyuridine. All three of these phenotypes are cotransducible. The tmk gene was mapped by cotransduction to approximately 30 min on the E. coli map.
摘要
大肠杆菌LD0181对每毫升15微克的2',3'-双脱氧胸苷敏感。经亚硝基胍诱变后，分离出一种衍生物，它在30℃时对每毫升40微克的同一化学物质具有抗性，并且丧失了在42℃的富集培养基上生长的能力。这个突变体TD105产生的胸苷酸激酶比亲本菌株的比活性低25倍，对胸苷酸的Km值高5倍。TD105中的胸苷酸池比亲本高4.4倍。除了温度敏感性和对2',3'-双脱氧胸苷的抗性外，该突变体对5-溴-2'-脱氧尿苷表现出超敏感性。所有这三种表型都是共转导的。通过共转导将tmk基因定位在大肠杆菌染色体图谱上大约30分钟处。
相似文献
1
Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activity.一株缺乏胸苷酸激酶活性的大肠杆菌突变体的分离与鉴定。
J Bacteriol. 1984 Feb;157(2):440-4. doi: 10.1128/jb.157.2.440-444.1984.
2
Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues.正常和恶性组织中嘧啶核苷单磷酸激酶的特性分析
Cancer Res. 1977 Jun;37(6):1593-7.
3
Genetic mapping in Escherichia coli of tmk, the locus for dTMP kinase.胸苷酸激酶基因座tmk在大肠杆菌中的遗传定位
J Bacteriol. 1986 Dec;168(3):1457-8. doi: 10.1128/jb.168.3.1457-1458.1986.
4
Guanylate kinase from Escherichia coli B.
Methods Enzymol. 1978;51:473-82. doi: 10.1016/s0076-6879(78)51065-3.
5
Enzymatic Manufacture of Deoxythymidine-5'-Triphosphate with Permeable Intact Cells of E. coli Coexpressing Thymidylate Kinase and Acetate Kinase.利用共表达胸苷酸激酶和乙酸激酶的大肠杆菌可渗透完整细胞酶法生产脱氧胸苷-5'-三磷酸。
J Microbiol Biotechnol. 2015 Dec 28;25(12):2034-42. doi: 10.4014/jmb.1506.06020.
6
Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus.大肠杆菌胸苷酸激酶：相应tmk基因座的分子克隆、核苷酸序列及基因组织
J Bacteriol. 1996 May;178(10):2804-12. doi: 10.1128/jb.178.10.2804-2812.1996.
7
[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].[小麦蓝矮植原体胸苷酸激酶（tmk）基因的分离、原核表达及活性分析]
Wei Sheng Wu Xue Bao. 2008 Jun;48(6):739-44.
8
Human thymidylate kinase. Purification, characterization, and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemia.
J Biol Chem. 1977 Aug 25;252(16):5686-91.
9
Mutational analysis of UMP kinase from Escherichia coli.大肠杆菌尿苷一磷酸激酶的突变分析
J Bacteriol. 1998 Feb;180(3):473-7. doi: 10.1128/JB.180.3.473-477.1998.
10
[Isolation and various properties of nucleoside monophosphate kinases from Escherichia coli].
Prikl Biokhim Mikrobiol. 1988 May-Jun;24(3):310-8.
引用本文的文献
1
Phage S144, A New Polyvalent Phage Infecting spp. and .噬菌体 S144，一种新的多价噬菌体，感染 和 。
Int J Mol Sci. 2020 Jul 22;21(15):5196. doi: 10.3390/ijms21155196.
2
Construction and complementation of in-frame deletions of the essential Escherichia coli thymidylate kinase gene.大肠杆菌必需胸苷酸激酶基因框内缺失的构建与互补
Appl Environ Microbiol. 2006 Feb;72(2):1288-94. doi: 10.1128/AEM.72.2.1288-1294.2006.
3
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
4
Substitution of an alanine residue for glycine 146 in TMP kinase from Escherichia coli is responsible for bacterial hypersensitivity to bromodeoxyuridine.将大肠杆菌中TMP激酶的甘氨酸146替换为丙氨酸残基会导致细菌对溴脱氧尿苷高度敏感。
J Bacteriol. 1998 Aug;180(16):4291-3. doi: 10.1128/JB.180.16.4291-4293.1998.
5
Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus.大肠杆菌胸苷酸激酶：相应tmk基因座的分子克隆、核苷酸序列及基因组织
J Bacteriol. 1996 May;178(10):2804-12. doi: 10.1128/jb.178.10.2804-2812.1996.
6
Isolation and characterization of an Escherichia coli K-12 mutant deficient in glutaredoxin.一种谷氧还蛋白缺陷型大肠杆菌K-12突变体的分离与鉴定
J Bacteriol. 1988 Jan;170(1):308-15. doi: 10.1128/jb.170.1.308-315.1988.
7
Genetic mapping in Escherichia coli of tmk, the locus for dTMP kinase.胸苷酸激酶基因座tmk在大肠杆菌中的遗传定位
J Bacteriol. 1986 Dec;168(3):1457-8. doi: 10.1128/jb.168.3.1457-1458.1986.
8
Linkage map of Escherichia coli K-12, edition 8.大肠杆菌K-12连锁图谱，第8版。
Microbiol Rev. 1990 Jun;54(2):130-97. doi: 10.1128/mr.54.2.130-197.1990.
本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Purification and properties of a pyrimidine deoxyriboside phosphorylase from Escherichia coli.来自大肠杆菌的嘧啶脱氧核糖核苷磷酸化酶的纯化及性质
Biochim Biophys Acta. 1958 Jun;28(3):562-6. doi: 10.1016/0006-3002(58)90519-5.
4
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
5
Isolation and properties of Tn10 insertions in the rac locus of Escherichia coli.大肠杆菌rac基因座中Tn10插入片段的分离与特性
Mol Gen Genet. 1981;183(2):333-40. doi: 10.1007/BF00270637.
6
P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12.大肠杆菌K-12的rac+和rac菌株中trg基因座的P1转导作图
J Bacteriol. 1982 Feb;149(2):529-33. doi: 10.1128/jb.149.2.529-533.1982.
7
Deoxycytidine triphosphate deaminase: characterization of an Escherichia coli mutant deficient in the enzyme.脱氧胞苷三磷酸脱氨酶：大肠杆菌中该酶缺陷型突变体的特性研究
J Bacteriol. 1971 Feb;105(2):666-72. doi: 10.1128/jb.105.2.666-672.1971.
8
A model for three-point analysis of random general transduction.随机普遍转导的三点分析模型
Genetics. 1966 Aug;54(2):405-10. doi: 10.1093/genetics/54.2.405.
9
10
DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells.核苷酸可通透的大肠杆菌细胞中的DNA合成。I. 经乙醚处理的细胞的制备及特性
J Mol Biol. 1971 Jun 28;58(3):739-53. doi: 10.1016/0022-2836(71)90037-4.
Zotero 插件