Weder D, Radsak K D
J Gen Virol. 1983 Dec;64 ( Pt 12):2749-61. doi: 10.1099/0022-1317-64-12-2749.
Analysis of chromatin preparations from [3H]glucosamine-labelled human foreskin fibroblasts revealed that a chromatin-associated glycopolypeptide with the approximate mol. wt. 130 000 (130K) is induced in response to either infection with human cytomegalovirus (HCMV) or serum treatment. Comparative limited proteolysis suggested that the [3H]glucosamine-labelled 130K polypeptides induced by these different stimuli were not identical. This observation was in contrast to results obtained by immunoprecipitation with antisera raised against the 130K glycopolypeptide from serum-induced cells which favoured a relatedness to the 130K polypeptide from virus-infected cultures. Two-dimensional separation by isoelectric focusing and SDS-polyacrylamide gel electrophoresis subsequently showed that the 130K glycopolypeptide from serum-induced cells consists of two components, one of which is identical to the single component observed in samples from HCMV-infected cultures. Experiments on the effect of glycosylation inhibitors on DNA replication in HCMV-infected as well as in serum-induced cells support the view that the host-specific chromatin-associated glycopolypeptide may be involved in DNA replication in infected cells.
对来自[³H]葡萄糖胺标记的人包皮成纤维细胞的染色质制剂进行分析发现，一种分子量约为130000（130K）的与染色质相关的糖多肽，在受到人巨细胞病毒（HCMV）感染或血清处理时会被诱导产生。比较性的有限蛋白酶解表明，由这些不同刺激诱导产生的[³H]葡萄糖胺标记的130K多肽并不相同。这一观察结果与用针对血清诱导细胞中130K糖多肽产生的抗血清进行免疫沉淀所得到的结果相反，后者表明其与病毒感染培养物中的130K多肽具有相关性。随后通过等电聚焦和SDS-聚丙烯酰胺凝胶电泳进行的二维分离显示，血清诱导细胞中的130K糖多肽由两个组分组成，其中一个与在HCMV感染培养物样品中观察到的单个组分相同。关于糖基化抑制剂对HCMV感染细胞以及血清诱导细胞中DNA复制影响的实验支持了这样一种观点，即宿主特异性的与染色质相关的糖多肽可能参与了感染细胞中的DNA复制。
