Radsak K, Mertensmaier I, Kaiser C, Wagner C
Arch Virol. 1985;85(3-4):217-30. doi: 10.1007/BF01314233.
Human cytomegalovirus is shown to induce in phosphonoacetic acid-treated human fibroblasts glycosylation of five polypeptides with approximate molecular weights of 200-250, 150, 135, 130 and 100 kilodaltons (kd). Except for the 130 kd product, these glycopolypeptides (gp) separate with the cytoplasmic fraction, only one (200-250 kd) with the chromatin fraction as well. The gp of 135 and 100 kd were found to be virus-specified as determined by immunoblotting and immunoprecipitation. The gp of 200-250 kd exhibited an immunological relatedness to fibronectin and are therefore considered host-specific products. Both subsets of gp participate in virus-induced surface membrane alterations as documented by living cell immunofluorescence.
已表明人巨细胞病毒能在经膦甲酸处理的人成纤维细胞中诱导5种多肽发生糖基化，这些多肽的分子量约为200 - 250、150、135、130和100千道尔顿（kd）。除了130 kd的产物外，这些糖多肽（gp）与细胞质部分一起分离，只有一种（200 - 250 kd）也与染色质部分一起分离。通过免疫印迹和免疫沉淀确定，135和100 kd的gp是病毒特异性的。200 - 250 kd的gp与纤连蛋白表现出免疫相关性，因此被认为是宿主特异性产物。如活细胞免疫荧光所示，这两个gp亚群都参与病毒诱导的表面膜改变。
