大肠杆菌K-12中的一般重组：RecBC酶的体内作用
General recombination in Escherichia coli K-12: in vivo role of RecBC enzyme.
作者信息
Yancey S D, Porter R D
出版信息
J Bacteriol. 1985 Apr;162(1):29-34. doi: 10.1128/jb.162.1.29-34.1985.
Abstract
Heterozygous lacZ- merodiploids of Escherichia coli K-12 have been used to study the role of the RecBC enzyme in general recombination. The transcribable intermediate assay detects the product of early steps in recombination without requiring the formation of viable recombinant colonies. Recombination is initiated by infection with lambda precA+. We have found that transcribable intermediate formation in crosses between F42 lac and chromosomal lac is dependent on F fertility functions and an active RecBC enzyme. Thus, the products of the recB and recC genes are required in early steps of recombination between these two substrates. Introduction of the F42 lac donor DNA by conjugation immediately after infection with lambda precA+ abolishes the requirement for an active RecBC enzyme.
摘要
大肠杆菌K-12的杂合lacZ-部分二倍体已被用于研究RecBC酶在一般重组中的作用。可转录中间体检测法可检测重组早期步骤的产物，而无需形成有活力的重组菌落。重组由λprecA+感染引发。我们发现，F42 lac与染色体lac之间杂交时可转录中间体的形成依赖于F育性功能和活性RecBC酶。因此，recB和recC基因的产物是这两种底物之间重组早期步骤所必需的。在用λprecA+感染后立即通过接合引入F42 lac供体DNA，消除了对活性RecBC酶的需求。
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