PMAP、PMAPS：DNA物理图谱构建程序。

PMAP, PMAPS: DNA physical map constructing programs.

作者信息

Polner G, Dorgai L, Orosz L

出版信息

Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):227-36. doi: 10.1093/nar/12.1part1.227.

DOI:10.1093/nar/12.1part1.227

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320999/

Abstract

Computer programs are described, which facilitate the construction of the restriction site (physical) map of DNA molecules. By knowing the length of each fragment and its degree of error in the single and the double restriction enzyme digestions, the programs give all the possibilities for the physical map. This method is applicable to linear DNA molecules. Several examples are presented which indicate the high efficiency of the programs in constructing restriction site maps for the 62 Kb chromosome of bacteriophage 16-3. We have constructed complex maps (i.e. EcoRI map with 16 and EcoRV with 11 fragments).

摘要

描述了一些计算机程序，这些程序有助于构建DNA分子的限制酶切位点（物理）图谱。通过了解每个片段的长度及其在单酶切和双酶切中的误差程度，这些程序给出了物理图谱的所有可能性。该方法适用于线性DNA分子。给出了几个例子，表明这些程序在构建噬菌体16 - 3的62 Kb染色体的限制酶切位点图谱方面具有很高的效率。我们已经构建了复杂的图谱（即含有16个片段的EcoRI图谱和含有11个片段的EcoRV图谱）。

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本文引用的文献

1

Automatic construction of restriction site maps.限制酶切位点图谱的自动构建。

Nucleic Acids Res. 1982 Jan 11;10(1):217-27. doi: 10.1093/nar/10.1.217.

2

Least-squares method for restriction mapping.用于限制酶切图谱分析的最小二乘法

Gene. 1978 Oct;4(2):167-74. doi: 10.1016/0378-1119(78)90028-8.

3

Elution of DNA from agarose gels after electrophoresis.电泳后从琼脂糖凝胶中洗脱DNA。

Methods Enzymol. 1979;68:176-82. doi: 10.1016/0076-6879(79)68012-6.

4

Gel electrophoresis of restriction fragments.限制性片段的凝胶电泳。

Methods Enzymol. 1979;68:152-76. doi: 10.1016/0076-6879(79)68011-4.

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