多聚体tRNA基因的转录
Transcription of multimeric tRNA genes.
作者信息
Ciliberto G, Traboni C, Cortese R
出版信息
Nucleic Acids Res. 1984 Jan 25;12(2):1277-85. doi: 10.1093/nar/12.2.1277.
Abstract
We have constructed a set of plasmids carrying a tRNAPro gene from C. elegans in a head to tail dimeric and trimeric arrangement with virtually no spacer sequence. We show that in two different transcriptional systems, each coding region functions as an internal promoter directing the synthesis of independent transcriptional products. This is in contrast with the property of natural head to tail dimeric arrangements found in yeast, where only one coding region functions as promoter (Mao et al., 1980 Cell 20, 589; Schmidt et al., 1980 Nature 287, 750). The evolutionary significance of our finding is discussed.
摘要
我们构建了一组质粒，其中携带来自秀丽隐杆线虫的tRNAPro基因，呈头对头二聚体和三聚体排列，几乎没有间隔序列。我们表明，在两种不同的转录系统中，每个编码区都作为一个内部启动子发挥作用，指导独立转录产物的合成。这与酵母中天然的头对头二聚体排列的特性形成对比，在酵母中只有一个编码区作为启动子发挥作用（Mao等人，1980年，《细胞》20卷，589页；Schmidt等人，1980年，《自然》287卷，750页）。我们讨论了这一发现的进化意义。
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本文引用的文献
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Dimeric transfer RNA precursors in S. pombe.粟酒裂殖酵母中的二聚体转运RNA前体。
Cell. 1980 Sep;21(2):509-16. doi: 10.1016/0092-8674(80)90488-2.
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A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.5S RNA基因中心的一个控制区域指导转录的特异性起始：I. 该区域的5'边界。
Cell. 1980 Jan;19(1):13-25. doi: 10.1016/0092-8674(80)90384-0.
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A split promoter for a eucaryotic tRNA gene.一种真核生物tRNA基因的分裂启动子。
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Transcription initiation of eucaryotic transfer RNA genes.真核生物转运RNA基因的转录起始
Cell. 1982 May;29(1):3-5. doi: 10.1016/0092-8674(82)90083-6.
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Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.体内tRNA基因的转录：单链模板与双链模板的比较
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4147-51. doi: 10.1073/pnas.77.7.4147.
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Relationship between the two components of the split promoter of eukaryotic tRNA genes.真核生物tRNA基因分裂启动子两个组分之间的关系。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1921-5. doi: 10.1073/pnas.79.6.1921.
7
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.真核生物tRNAPro基因的启动子由三个不连续的区域组成。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1195-9. doi: 10.1073/pnas.79.4.1195.
8
Transcription by RNA polymerase III.由RNA聚合酶III进行转录。
Curr Top Dev Biol. 1983;18:59-88. doi: 10.1016/s0070-2153(08)60579-7.
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Cell. 1983 Mar;32(3):725-33. doi: 10.1016/0092-8674(83)90058-2.
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