多聚体tRNA基因的转录

Ciliberto G, Traboni C, Cortese R

Nucleic Acids Res. 1984 Jan 25;12(2):1277-85. doi: 10.1093/nar/12.2.1277.

We have constructed a set of plasmids carrying a tRNAPro gene from C. elegans in a head to tail dimeric and trimeric arrangement with virtually no spacer sequence. We show that in two different transcriptional systems, each coding region functions as an internal promoter directing the synthesis of independent transcriptional products. This is in contrast with the property of natural head to tail dimeric arrangements found in yeast, where only one coding region functions as promoter (Mao et al., 1980 Cell 20, 589; Schmidt et al., 1980 Nature 287, 750). The evolutionary significance of our finding is discussed.

我们构建了一组质粒，其中携带来自秀丽隐杆线虫的tRNAPro基因，呈头对头二聚体和三聚体排列，几乎没有间隔序列。我们表明，在两种不同的转录系统中，每个编码区都作为一个内部启动子发挥作用，指导独立转录产物的合成。这与酵母中天然的头对头二聚体排列的特性形成对比，在酵母中只有一个编码区作为启动子发挥作用（Mao等人，1980年，《细胞》20卷，589页；Schmidt等人，1980年，《自然》287卷，750页）。我们讨论了这一发现的进化意义。