Snyder H W, Singhal M C, Zuckerman E E, Hardy W D
Virology. 1984 Jan 15;132(1):205-10. doi: 10.1016/0042-6822(84)90104-1.
A new strain of feline sarcoma virus, designated HZ1-FeSV, was isolated from a 4-year-old domestic cat with multicentric fibrosarcoma. A primary tumor cell line was established and virus produced from that line was found to induce foci in feline embryonic lung fibroblasts (FLF3) and mink lung fibroblasts (CCL64) in tissue culture and fibrosarcomas in inoculated 10-week-old kittens. The derivation of transformed nonproducer clones of FLF3 and CCL64 cells containing helper virus-rescuable, focus-forming activity indicated that HZ1-FeSV was defective for replication. The only discernible translation product of the HZ1-FeSV genome in cultured cells was a 100,000-Da polyprotein (P100) which contained amino-terminal sequences of the FeLV gag gene precursor protein covalently linked to a sarcoma virus-specific domain. Immunoprecipitates containing P100 exhibited a protein kinase activity capable of phosphorylating tyrosine residues of P100. Immunologically, P100 was highly cross-reactive with gag-fes polyproteins encoded by two previously characterized strains of FeSV, the GA- and the ST-FeSV. By comparison of methionine-containing tryptic peptides, the HZ1-FeSV protein was shown to be more closely related to the GA-FeSV protein than to the ST-FeSV protein, but to be distinguishable from both other proteins.
一种新的猫肉瘤病毒株，命名为HZ1-FeSV，是从一只患有多中心纤维肉瘤的4岁家猫身上分离出来的。建立了一个原发性肿瘤细胞系，发现从该细胞系产生的病毒在组织培养中能在猫胚胎肺成纤维细胞（FLF3）和貂肺成纤维细胞（CCL64）中诱导病灶，并能在接种的10周龄小猫中诱发纤维肉瘤。含有可拯救辅助病毒、形成病灶活性的FLF3和CCL64细胞转化非生产性克隆的衍生表明HZ1-FeSV在复制方面存在缺陷。在培养细胞中，HZ1-FeSV基因组唯一可识别的翻译产物是一种100,000道尔顿的多聚蛋白（P100），它包含与肉瘤病毒特异性结构域共价连接的FeLV gag基因前体蛋白的氨基末端序列。含有P100的免疫沉淀物表现出一种能够磷酸化P100酪氨酸残基的蛋白激酶活性。在免疫学上，P100与由两种先前鉴定的FeSV毒株GA-FeSV和ST-FeSV编码的gag-fes多聚蛋白具有高度交叉反应性。通过对含甲硫氨酸的胰蛋白酶肽段的比较，发现HZ1-FeSV蛋白与GA-FeSV蛋白的关系比与ST-FeSV蛋白的关系更密切，但与其他两种蛋白都有区别。
