Roebroek A J, Schalken J A, Onnekink C, Bloemers H P, Van de Ven W J
J Virol. 1987 Jun;61(6):2009-16. doi: 10.1128/JVI.61.6.2009-2016.1987.
The nucleotide sequence of the feline c-fes/fps proto-oncogene was analyzed. Comparison with v-fes and v-fps revealed that all v-fes/fps homologous sequences were dispersed over 11 kilobase pairs in 19 interspersed segments. All segments, numbered exon 1 to exon 19 as in the chicken and human loci, were flanked by consensus splice junctions. The putative promoter region contained a CATT sequence and three CCGCCC motifs which were also found in the human locus at similar positions. About 200 nucleotides downstream of a translational stop codon in exon 19, a putative poly(A) addition signal was identified. Using the putative translation initiation codon in exon 2, a 93,000-molecular-weight protein could be deduced. This protein resembled very well the putative protein of the human c-fes/fps proto-oncogene (94% overall homology) and, although less well, the putative protein of the chicken c-fes/fps proto-oncogene (70% overall homology). As far as the feline c-fes/fps proto-oncogene sequences transduced to the Gardner-Arnstein (GA) and Snyder-Theilen (ST) strains of feline sarcoma virus (FeSV) are concerned, homology in deduced amino acid sequences between the GA- and ST-v-fes viral oncogenes and the proto-oncogene was 99%. Analysis of the recombination junctions between feline leukemia virus and v-fes sequences in GA- and ST-FeSV proviral DNA revealed for the left-hand junction the involvement of homologous recombination, presumably at the DNA level. The right-hand junction, which appeared identical in the GA-FeSV and ST-FeSV genomes, could have been the result of a site-specific recombination at the RNA level.
对猫科动物c-fes/fps原癌基因的核苷酸序列进行了分析。与v-fes和v-fps的比较显示，所有v-fes/fps同源序列分散在11千碱基对的19个散布片段中。所有片段，按照鸡和人类基因座中的编号方式从外显子1到外显子19进行编号，两侧均为共有剪接连接。推测的启动子区域包含一个CATT序列和三个CCGCCC基序，在人类基因座的相似位置也发现了这些基序。在外显子19中翻译终止密码子下游约200个核苷酸处，鉴定出一个推测的聚腺苷酸化信号。使用外显子2中的推测翻译起始密码子，可以推导得到一种分子量为93,000的蛋白质。该蛋白质与人类c-fes/fps原癌基因的推测蛋白质非常相似（总体同源性为94%），与鸡c-fes/fps原癌基因的推测蛋白质的相似性稍低（总体同源性为70%）。就转导至猫肉瘤病毒（FeSV）的加德纳 - 阿恩斯坦（GA）和斯奈德 - 泰伦（ST）毒株的猫科动物c-fes/fps原癌基因序列而言，GA - 和ST - v-fes病毒癌基因与原癌基因之间推导氨基酸序列的同源性为99%。对GA - 和ST - FeSV前病毒DNA中猫白血病病毒与v-fes序列之间的重组连接点进行分析，结果表明左手边的连接点涉及同源重组，推测发生在DNA水平。右手边的连接点在GA - FeSV和ST - FeSV基因组中看起来相同，可能是RNA水平上位点特异性重组的结果。
