哺乳期大鼠乳腺高尔基体膜的孔特性。pH值和温度的影响以及重组入磷脂囊泡的情况。
Pore properties of the Golgi membrane from lactating-rat mammary gland. Effects of pH and temperature and reconstitution into phospholipid vesicles.
作者信息
White M D, Ward S, Kuhn N J
出版信息
Biochem J. 1984 Jan 1;217(1):297-301. doi: 10.1042/bj2170297.
Abstract
Golgi-membrane vesicle penetration was studied by osmotic lysis. Pronounced temperature dependence of mannitol and mannoheptitol penetration over 0-37 degrees C gave linear Arrhenius plots, with activation energies of 75 and 117 kJ/mol respectively. Glucose penetration was constant over pH 5-9, but was respectively faster and slower at higher and lower pH values. Solubilized, dialysed, heat-stable extracts of Golgi membrane were reconstituted into egg yolk phospholipid membranes with apparent recovery of specific permeability. Penetration is interpreted in terms of a pore, for which the Renkin equation predicts a radius of about 0.54 nm.
摘要
通过渗透裂解研究了高尔基体膜囊泡的穿透情况。在0至37摄氏度范围内，甘露醇和甘露庚醇的穿透表现出明显的温度依赖性，得到线性的阿伦尼乌斯图，活化能分别为75和117 kJ/mol。葡萄糖的穿透在pH 5至9范围内保持恒定，但在更高和更低的pH值下分别更快和更慢。将溶解、透析、热稳定的高尔基体膜提取物重构成蛋黄磷脂膜，特异性通透性明显恢复。穿透情况用孔来解释，根据伦金方程预测该孔的半径约为0.54纳米。
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