White M D, Kuhn N J, Ward S
Biochem J. 1980 Sep 15;190(3):621-4. doi: 10.1042/bj1900621.
The Golgi-membrane vesicles present in particulate preparations of lactating rat mammary gland were biosynthetically loaded with [14C]lactose. This lactose was effectively retained by particles sedimented after exposure to 0.25 M-disaccharide, but was partly lost after exposure to 0.25 M-glucose or other solutes of similar size. Loss of lactose was time-, concentration- and temperature-dependent and varied with the solute structure. This behaviour is ascribed to the presence of protein in the Golgi membrane, forming a specific carrier or channel that serves to supply glucose for lactose synthesis.
在泌乳大鼠乳腺的微粒制剂中存在的高尔基体膜囊泡通过生物合成方式装载了[14C]乳糖。在暴露于0.25M二糖后沉淀的颗粒有效地保留了这种乳糖，但在暴露于0.25M葡萄糖或其他大小相似的溶质后部分乳糖会丢失。乳糖的丢失是时间、浓度和温度依赖性的，并且随溶质结构而变化。这种行为归因于高尔基体膜中存在蛋白质，形成了一个特定的载体或通道，用于为乳糖合成提供葡萄糖。
