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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

White M D, Ward S, Kuhn N J

Biochem J. 1981 Dec 15;200(3):663-9. doi: 10.1042/bj2000663.

Golgi membrane vesicles, isolated from lactating-rat mammary gland and greatly enriched in galactosyltransferase (EC 2.4.1.22), contained over 40 separate bands of protein, including some periodic acid)(Schiff-staining material and free thiol groups, when analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 2. The membrane lipids were enriched in phosphatidylcholine, phosphatidylethanolamine and unesterified cholesterol. 3. Membrane fluidity, as monitored by the fluorescence polarization of 1,6-diphenylhexa-1,3,5-triene, increased linearly over 5-37 degrees C. 4. The vesicle membranes were impermeable to lactose over a wide pH range, but admitted electrolytes of molecular weight below about 300. 5. These properties are discussed with respect to other cellular membranes and the secretion of milk products.

从泌乳大鼠乳腺中分离出的高尔基体膜囊泡，富含半乳糖基转移酶（EC 2.4.1.22），通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析时，含有40多条不同的蛋白带，包括一些经高碘酸-希夫染色的物质和游离巯基。2. 膜脂富含磷脂酰胆碱、磷脂酰乙醇胺和未酯化胆固醇。3. 通过1,6-二苯基己-1,3,5-三烯的荧光偏振监测，膜流动性在5至37摄氏度范围内呈线性增加。4. 囊泡膜在很宽的pH范围内对乳糖不可渗透，但能允许分子量低于约300的电解质通过。5. 就其他细胞膜和奶制品的分泌对这些特性进行了讨论。

White M D, Ward S, Kuhn N J

Biochem J. 1981 Dec 15;200(3):663-9. doi: 10.1042/bj2000663.

Golgi membrane vesicles, isolated from lactating-rat mammary gland and greatly enriched in galactosyltransferase (EC 2.4.1.22), contained over 40 separate bands of protein, including some periodic acid)(Schiff-staining material and free thiol groups, when analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 2. The membrane lipids were enriched in phosphatidylcholine, phosphatidylethanolamine and unesterified cholesterol. 3. Membrane fluidity, as monitored by the fluorescence polarization of 1,6-diphenylhexa-1,3,5-triene, increased linearly over 5-37 degrees C. 4. The vesicle membranes were impermeable to lactose over a wide pH range, but admitted electrolytes of molecular weight below about 300. 5. These properties are discussed with respect to other cellular membranes and the secretion of milk products.

从泌乳大鼠乳腺中分离出的高尔基体膜囊泡，富含半乳糖基转移酶（EC 2.4.1.22），通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析时，含有40多条不同的蛋白带，包括一些经高碘酸-希夫染色的物质和游离巯基。2. 膜脂富含磷脂酰胆碱、磷脂酰乙醇胺和未酯化胆固醇。3. 通过1,6-二苯基己-1,3,5-三烯的荧光偏振监测，膜流动性在5至37摄氏度范围内呈线性增加。4. 囊泡膜在很宽的pH范围内对乳糖不可渗透，但能允许分子量低于约300的电解质通过。5. 就其他细胞膜和奶制品的分泌对这些特性进行了讨论。