无法结合表皮生长因子的3T3变体在共培养中不能互补。
3T3 variants unable to bind epidermal growth factor cannot complement in co-culture.
作者信息
Terwilliger E, Herschman H R
出版信息
Biochem Biophys Res Commun. 1984 Jan 13;118(1):60-4. doi: 10.1016/0006-291x(84)91067-2.
PMID:6320832
Abstract
A Swiss-Webster 3T3 variant, called 3T3-ENR7, unable to divide in response to epidermal growth factor was isolated by the mitogen-colchicine selection technique. Like the other EGF non-proliferative variants (3T3-NR6 and 3T3-TNR2), 3T3-ENR7 was unable to bind 125I-EGF. Pairwise co-culture of the three independently isolated EGF non-responsive variants did not restore mitogenic responsiveness to EGF.
摘要
通过丝裂原 - 秋水仙碱选择技术分离出一种瑞士 - 韦伯斯特3T3变体，称为3T3 - ENR7，它不能对表皮生长因子作出分裂反应。与其他表皮生长因子非增殖变体（3T3 - NR6和3T3 - TNR2）一样，3T3 - ENR7不能结合125I - 表皮生长因子。对这三种独立分离的对表皮生长因子无反应的变体进行两两共培养，并未恢复对表皮生长因子的促有丝分裂反应。
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