脊髓灰质炎病毒的蛋白质加工图谱。
Protein processing map of poliovirus.
作者信息
Pallansch M A, Kew O M, Semler B L, Omilianowski D R, Anderson C W, Wimmer E, Rueckert R R
出版信息
J Virol. 1984 Mar;49(3):873-80. doi: 10.1128/JVI.49.3.873-880.1984.
Abstract
Five previously unmapped proteins (5a, 7d, 8, 9b, and 10) were located on the proteolytic processing map of the polyprotein. One of the proteins, 9b, appears to be the sister fragment of a cleavage reaction (P3-9 leads to P3-9b + VPg). Two of the other newly mapped proteins, 8 and 10, have been identified as sister fragments of X-related proteins 3b and 5b; thus, P2-3b leads to P2-8 + P2-5b and P2-5b leads to P2-10 + P2-X. The remaining proteins, 5a and 7d, mapped in the 1b protein and appear to result from the cleavages P3-1b leads to P3-5a + P3-6b and P3-4b leads to P3-7d + P3-6b. These assignments account for over 95% of the total polioviral proteins and complete the mapping of the major processing pathways.
摘要
五种先前未定位的蛋白质（5a、7d、8、9b和10）位于多聚蛋白的蛋白水解加工图谱上。其中一种蛋白质9b似乎是切割反应（P3 - 9产生P3 - 9b + VPg）的姐妹片段。另外两个新定位的蛋白质8和10，已被鉴定为X相关蛋白质3b和5b的姐妹片段；因此，P2 - 3b产生P2 - 8 + P2 - 5b，P2 - 5b产生P2 - 10 + P2 - X。其余的蛋白质5a和7d定位于1b蛋白中，似乎是由P3 - 1b产生P3 - 5a + P3 - 6b以及P3 - 4b产生P3 - 7d + P3 - 6b的切割反应所致。这些定位解释了超过95%的脊髓灰质炎病毒总蛋白，并完成了主要加工途径的图谱绘制。
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