Semler B L, Anderson C W, Kitamura N, Rothberg P G, Wishart W L, Wimmer E
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3464-8. doi: 10.1073/pnas.78.6.3464.
A partial amino-terminal amino acid sequence of each of the major proteins encoded by the replicase region (P3) of the poliovirus genome has been determined. A comparison of this sequence information with the amino acid sequence predicted from the RNA sequence that has been determined for the 3' region of the poliovirus genome has allowed us to locate precisely the proteolytic cleavage sites at which the initial polyprotein is processed to create the poliovirus products P3-1b (NCVP1b), P3-2 (NCVP2), P3-4b (NCVP4b), and P3-7c (NCVP7c). For each of these products, as well as for the small genome-linked protein VPg, proteolytic cleavage occurs between a glutamine and a glycine residue to create the amino terminus of each protein. This result suggests that a single proteinase may be responsible for all of these cleavages. The sequence data also allow the precise positioning of the genome-linked protein VPg within the precursor P3-1b just proximal to the amino terminus of polypeptide P3-2.
已确定脊髓灰质炎病毒基因组复制酶区域（P3）编码的每种主要蛋白质的部分氨基末端氨基酸序列。将该序列信息与根据已确定的脊髓灰质炎病毒基因组3'区域RNA序列预测的氨基酸序列进行比较，使我们能够精确地定位蛋白水解切割位点，在这些位点处，初始多聚蛋白被加工以产生脊髓灰质炎病毒产物P3-1b（NCVP1b）、P3-2（NCVP2）、P3-4b（NCVP4b）和P3-7c（NCVP7c）。对于这些产物中的每一种以及小基因组连接蛋白VPg，蛋白水解切割发生在谷氨酰胺和甘氨酸残基之间，以形成每种蛋白质的氨基末端。这一结果表明，单一蛋白酶可能负责所有这些切割。序列数据还允许将基因组连接蛋白VPg精确地定位在前体P3-1b中，就在多肽P3-2氨基末端的近端。
