减毒脊髓灰质炎病毒萨宾1型毒株基因组的完整核苷酸序列。
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
作者信息
Nomoto A, Omata T, Toyoda H, Kuge S, Horie H, Kataoka Y, Genba Y, Nakano Y, Imura N
出版信息
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5793-7. doi: 10.1073/pnas.79.19.5793.
Abstract
The complete nucleotide sequence of the genome of the type 1 poliovirus vaccine strain (LSc,2ab) was determined by using molecular cloning and rapid sequence analysis techniques. The restriction fragments of double-stranded cDNA synthesized from the vaccine strain RNA were inserted into the adequate sites of cloning vector pBR322. Sequence analysis of the cloned DNAs revealed that the virion RNA molecule was 7,441 nucleotides long and polyadenylylated at the 3' terminus. When the nucleotide sequence was compared with that of the genome of the virulent parental strain (Mahoney), 57 base substitutions were observed to be scattered all over the genome. Of these, 21 resulted in amino acid changes in a number of viral proteins. A cluster of amino acid changes is located in the viral coat proteins, especially in the NH2-terminal half of the viral capsid protein VP1. These results may imply that the mutations in the VP1 coding region contribute to attenuation.
摘要
利用分子克隆和快速序列分析技术测定了1型脊髓灰质炎疫苗株（LSc，2ab）基因组的完整核苷酸序列。从疫苗株RNA合成的双链cDNA的限制性片段被插入到克隆载体pBR322的适当位点。对克隆DNA的序列分析表明，病毒粒子RNA分子长7441个核苷酸，3'末端有多聚腺苷酸化。当将核苷酸序列与强毒株亲本株（Mahoney）的基因组序列进行比较时，发现57个碱基替换分散在整个基因组中。其中，21个导致多种病毒蛋白的氨基酸发生变化。一组氨基酸变化位于病毒衣壳蛋白中，特别是在病毒衣壳蛋白VP1的NH2末端一半区域。这些结果可能意味着VP1编码区的突变有助于减毒。
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