豚鼠因苯妥英钠导致实验性昏迷时的脑氨基酸模式。

The brain amino acid pattern in experimental coma due to phenytoin in guinea pigs.

作者信息

Sabato A F, Pala F, Sammartino M, Barbi S, Beccia F

出版信息

Resuscitation. 1984 Feb;11(1-2):123-5. doi: 10.1016/0300-9572(84)90040-6.

Abstract

An experimental study was carried out on 50 guinea pigs to evaluate the amino acid pattern in the whole brain after a toxic dose of phenytoin (1 g . kg-1 body wt). One group of 25 guinea pigs was treated with the drug which made them comatose, and their brains removed by craniotomy and frozen in liquid nitrogen; 25 guinea pigs were used as a control group. The brain amino acid pattern was determined by ion-exchange chromatography. All the amino acids, except threonine and methionine, decreased. Hypotheses about the particular cerebral metabolic pathways involved are discussed.

摘要

对50只豚鼠进行了一项实验研究，以评估在给予苯妥英钠毒性剂量（1 g·kg-1体重）后全脑的氨基酸模式。一组25只豚鼠用该药物处理，使其昏迷，然后通过开颅术取出大脑并在液氮中冷冻；25只豚鼠用作对照组。通过离子交换色谱法测定脑氨基酸模式。除苏氨酸和蛋氨酸外，所有氨基酸均减少。文中讨论了关于所涉及的特定脑代谢途径的假设。